Preparation of multiplexed control materials for cancer mutation analysis by genome editing in GM12878 cells,Journal of Clinical Laboratory Analysis

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Preparation of multiplexed control materials for cancer mutation analysis by genome editing in GM12878 cells
Journal of Clinical Laboratory Analysis ( IF 2.6 ) Pub Date : 2021-11-23 , DOI: 10.1002/jcla.24139
Guigao Lin _{1,

2,

3} , Kuo Zhang _{1,

2,

3} , Yanxi Han _{1,

3} , Rongxue Peng _{1,

3} , Jinming Li _{1,

2,

3}

Affiliation

Quality control materials are necessary for assay development, test validation, and proficiency testing in cancer mutation analysis. Most of the existing controls for somatic mutations only harbor a single variant and are derived from unstable cell lines. This study aimed to establish a method to create stable multianalyte controls in a defined background by genome editing in GM12878 cells, which also can be applied for the reference of next-generation sequencing.

中文翻译：

通过基因组编辑在 GM12878 细胞中制备用于癌症突变分析的多路复用对照材料

质量控制材料对于癌症突变分析中的检测开发、测试验证和能力测试是必需的。大多数现有的体细胞突变对照仅含有一个变体，并且来自不稳定的细胞系。本研究旨在建立一种方法，通过在 GM12878 细胞中进行基因组编辑，在特定背景下创建稳定的多分析物对照，该方法也可用于下一代测序的参考。

更新日期：2021-11-23

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