A solid 10: A 10-hydroxy-2-decenoic acid (10-HDA) synthesis pathway is designed using decanoic acid as the substrate via a novel two-step whole-cell catalytic reaction. Step 1 involves the modification of E. coli β-oxidation pathway to optimize the key enzymes of the trans- -2- dec enoic acid synthesis pathway. In step 2, a P450 terminal hydroxylase was designed to increase the trans- -2- dec enoic acid terminal hydroxylation, leading to 10-HDA synthesis.

中文翻译：

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在工程大肠杆菌中通过全细胞催化从癸酸生产 10-羟基-2-癸烯酸

固体 10 : 10-羟基-2-癸烯酸 (10-HDA) 合成途径是使用癸酸作为底物，通过新的两步全细胞催化反应设计的。第一步涉及对大肠杆菌β-氧化途径的修饰，以优化反式--2-癸烯酸合成途径的关键酶。在步骤 2 中，设计了 P450 末端羟化酶以增加反式- -2- 癸烯酸末端羟化，从而导致 10-HDA 合成。