ABSTRACT

The liposomal membrane transport assay, which uses 8-hydroxypyrene-1,3,6-trisulfonic acid to monitor the internal pH of the vesicles (the HPTS assay), is a widely used technique for analysing the activity of anionophore-facilitated transport across a phospholipid membrane. This paper describes the stepwise technique to conduct this transport assay, detailing both the perks and pitfalls of using this method to determine the activity of an anionophore and the transport mechanism.

中文翻译：

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超分子方法：8-羟基芘-1,3,6-三磺酸 (HPTS) 转运试验

摘要

脂质体膜转运试验使用 8-羟基芘-1,3,6-三磺酸来监测囊泡的内部 pH 值（HPTS 试验），是一种广泛使用的技术，用于分析阴离子载体促进转运的活性。磷脂膜。本文描述了进行这种运输测定的逐步技术，详细介绍了使用这种方法来确定阴离子载体的活性和运输机制的好处和缺陷。