Here, the semi-invasive direct electrochemical detection of melanoma biomarkers in non-treated skin has been envisaged. The enzyme tyrosinase (TYR) was to be addressed with a microneedle electrochemical sensor. The microneedles were fabricated by polydimethylsiloxane (PDMS) casting with stable polymers. The as-prepared microneedles (MNs) were then coated by gold sputtering. The gold MNs were finally covered by alginate/catechol to provide a liquid electrolyte layer that contained electroactive species, such as catechol, whose redox state can be linked to the concentration of TYR in the skin. The sensor showed high sensitivity of 7.52 µA·mg–1·mL TYR in dummy skin. A relative standard deviation (RSD) of 8.54 % (n=5) demonstrated that the Catechol@alginate: gold MN electrode had excellent reproducibility for continuous glucose detection. Also, five parallel Catechol@alginate: gold MN electrodes were fabricated using the same experimental setup and showed an RSD of 3.95 % (n=5). These results suggest that the electrode fabrication process was reproducible, and Catechol@alginate: gold MN biosensors demonstrated high stability for the repetitive detection of TYR. Furthermore, the sensor has high selectivity in the presence of interfering components towards TYR screening.

中文翻译：

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用于酪氨酸酶传感的透皮金注射微针的离体性能评估

在这里，设想了对未经处理的皮肤中黑色素瘤生物标志物的半侵入性直接电化学检测。酪氨酸酶 (TYR) 将使用微针电化学传感器进行处理。微针是通过聚二甲基硅氧烷 (PDMS) 浇铸与稳定聚合物制成的。然后通过金溅射涂覆所制备的微针（MN）。金 MNs 最终被海藻酸盐/儿茶酚覆盖，以提供含有电活性物质（例如儿茶酚）的液体电解质层，其氧化还原状态可与皮肤中 TYR 的浓度有关。该传感器在假人皮肤中显示出 7.52 µA·mg–1·mL TYR 的高灵敏度。8.54 % (n=5) 的相对标准偏差 (RSD) 表明儿茶酚@藻酸盐：金 MN 电极对于连续葡萄糖检测具有出色的重现性。还，五个平行的儿茶酚@藻酸盐：金 MN 电极使用相同的实验装置制造，其 RSD 为 3.95% (n=5)。这些结果表明电极制造过程是可重复的，并且儿茶酚@藻酸盐：金 MN 生物传感器对 TYR 的重复检测表现出高稳定性。此外，该传感器在存在干扰成分的情况下对 TYR 筛选具有高选择性。