Colanic Acid: Biosynthetic Overproduction by Engineering Escherichia coli and Physical Property Characterization,Journal of Agricultural and Food Chemistry

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Colanic Acid: Biosynthetic Overproduction by Engineering Escherichia coli and Physical Property Characterization
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-11-12 , DOI: 10.1021/acs.jafc.1c04823
Jun Qiao _{1,

2} , Yi Zhan _{1,

2} , Xin Tan _{1,

2} , Yuantao Liu ₃ , Xiaoqing Hu _{1,

4} , Xiaoyuan Wang _{1,

2,

4}

Affiliation

Colanic acid has promising applications in food, cosmetic, and healthcare fields. In this study, a recombinant WQM003/pRAU was constructed by deleting genes lon and hns and overexpressing genes rcsA and galU in E. coli MG1655Δ(L-Q). After systematic optimization of fermentation conditions, colanic acid yield in WQM003/pRAU reached 19.79 g/L, the highest yield reported so far. The colanic acid produced by WQM003/pRAU was purified and its structure and physical properties were determined. This colanic acid shows a triple-helical structure and is stable up to 102 °C, and its melting temperature is 253.9 °C. This colanic acid shows a sphere-like chain conformation in aqueous solution. The viscosity of this colanic acid solution is related to concentration, shear rate, salt, temperature, and pH. At high concentrations, this colanic acid shows both viscous and elastic behaviors. These results suggest that the colanic acid produced by WQM003/pRAU has broad application prospects.

中文翻译：

可拉酸：通过工程大肠杆菌和物理特性表征的生物合成过量生产

可兰酸在食品、化妆品和保健领域具有广阔的应用前景。本研究通过删除lon和hns基因并在大肠杆菌中过表达基因rcsA和galU构建重组 WQM003/pRAUMG1655Δ(LQ)。经过系统优化发酵条件，WQM003/pRAU中可拉酸产量达到19.79 g/L，是目前报道的最高产量。对WQM003/pRAU生产的可拉酸进行纯化并测定其结构和物理性质。这种可拉酸呈三螺旋结构，在高达 102°C 的温度下稳定，其熔点为 253.9°C。这种可拉酸在水溶液中显示出球形链构象。这种可拉酸溶液的粘度与浓度、剪切速率、盐分、温度和 pH 值有关。在高浓度下，这种可拉酸显示出粘性和弹性行为。这些结果表明WQM003/pRAU产生的可拉酸具有广阔的应用前景。

更新日期：2021-11-24

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