Closed photobioreactors are ideal for the production of high-purity and high-value microalgal and cyanobacterial products. However, dissolved oxygen accumulation in closed reactors is detrimental to the growth. Conventional aeration for oxygen removal comprises up to 40% of the total culture cost, and energy-efficient dissolved oxygen removal is demanded. In this study, a novel gas-permeable bag photobioreactor was developed by the incorporation of a gas-permeable film. A hydrophobic microporous film was the most effective for both oxygen permeability and cost compared to silicone or fluororesin. The microporous film was placed on the backside of the reactor as an irradiation reflector. In a culture of Euhalothece sp., less than half of the steady-state dissolved oxygen concentration was demonstrated in the gas-permeable reactor than a non-permeable reactor. The gas-permeable reactor exhibited an oxygen transfer coefficient of 0.084 min⁻¹ under continuous mixing, which is high enough to maintain approximately 300% dissolved oxygen concentration with 58 mg L⁻¹ h⁻¹ biomass productivity without aeration (equivalent to 1.4 g L⁻¹ d⁻¹ under 24-h light). The estimated energy requirement for the gas-permeable reactor can be less than 1% of the aerated reactor of the same size. These results suggested the effectiveness of the gas-permeable reactor for energy-efficient algal mass culture.

封闭式光生物反应器是生产高纯度和高价值的微藻和蓝藻产品的理想选择。然而，密闭反应器中溶解氧的积累不利于生长。用于除氧的常规曝气占总培养成本的 40%，并且需要节能的溶解氧去除。在这项研究中，通过结合透气膜开发了一种新型透气袋式光生物反应器。与有机硅或氟树脂相比，疏水性微孔薄膜在透氧性和成本方面最有效。微孔膜被放置在反应器的背面作为辐射反射器。在Euhalothece文化中sp.，与非渗透性反应器相比，气体渗透性反应器中的稳态溶解氧浓度不到一半。气体可渗透反应器在连续混合下表现出 0.084 min ^-1的氧转移系数，该系数足够高以保持约 300% 的溶解氧浓度和 58 mg L ^-1  h ^-1生物质生产率，无需曝气（相当于 1.4 g L ^-1 d ^-1在 24 小时光照下）。气体可渗透反应器的估计能量需求可以小于相同尺寸的曝气反应器的 1%。这些结果表明气体可渗透反应器对于节能藻类大规模培养的有效性。