Herein, we re-explored α-cyano-4-hydroxycinnamic acid (CHCA) as a reactive matrix for selective and sensitive analysis of glutathione (GSH) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). CHCA efficiently reacted with GSH, and the resulting CHCA-GSH conjugate was readily detected by MALDI-MS without interferences. The detection limit of the CHCA-GSH conjugate decreased to 200 pmol μL-1, which was 2 orders of magnitude lower than that of pure GSH.Forapplication, CHCA was successfully applied for the detection of GSH, present in HepG2 cell lysates. The results demonstrated detection advantages of simple, high-throughput, and selective and screening of GSH in biological samples by MALDI-MS.

中文翻译：

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重新探索 α-氰基-4-羟基肉桂酸作为反应基质，通过 MALDI-MS 选择性检测谷胱甘肽。

在此，我们重新探索了 α-氰基-4-羟基肉桂酸 (CHCA) 作为反应基质，通过基质辅助激光解吸/电离质谱 (MALDI-MS) 选择性和灵敏地分析谷胱甘肽 (GSH)。CHCA 与 GSH 有效反应，所得 CHCA-GSH 偶联物可通过 MALDI-MS 轻松检测，不受干扰。CHCA-GSH偶联物的检测限降低到200 pmol μL-1，比纯GSH低2个数量级。在应用方面，CHCA成功应用于检测HepG2细胞裂解物中的GSH。结果表明，MALDI-MS具有简单、高通量、选择性筛选生物样品中GSH的检测优势。