Fully automated light transmission aggregometry on a disc for platelet function tests,Lab on a Chip

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Fully automated light transmission aggregometry on a disc for platelet function tests
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-10-27 , DOI: 10.1039/d1lc00708d
Chi-Ju Kim _{1,

2} , Jungmin Kim _{1,

2} , Jonathan Sabaté Del Río ₂ , Dong Yeob Ki _{1,

2} , Junyoung Kim _{1,

2} , Yoon-Kyoung Cho _{1,

2}

Affiliation

Platelet function tests, a group of assays that measure the ability of platelets to aggregate and promote clotting in a sample of blood, are performed in various medical fields to assess inherited platelet function disorders and monitor antiplatelet therapies. Light transmission aggregometry (LTA) is considered the gold standard for platelet function assessment. However, the lack of a standardized protocol is a major drawback when applied at the point of care. Moreover, it is a time-consuming and labor-intensive assay that requires a large volume of blood. Here, we describe the design, fabrication, and operation of a centrifugal microfluidic disc that can perform a fully automated LTA assay from a small volume of a whole blood sample (<1 mL), achieving highly reproducible results (3.2% coefficient of variation) within a short period (<25 min). The assays performed with this device yield more precise and accurate results than traditional LTA because of the automation of the reaction steps, minimal human operation, robust detection strategy via the distinctive structure of the microfluidic chamber, and quick analysis that minimizes the adverse effects of platelet instability.

中文翻译：

用于血小板功能测试的光盘上的全自动光透射聚集测量

血小板功能测试是一组测定血小板在血液样本中聚集和促进凝血能力的检测，在各个医学领域进行，以评估遗传性血小板功能障碍并监测抗血小板治疗。光透射聚集法 (LTA) 被认为是血小板功能评估的金标准。然而，在护理点应用时，缺乏标准化协议是一个主要缺点。此外，它是一种耗时且费力的分析，需要大量的血液。在这里，我们描述了离心微流体盘的设计、制造和操作，该盘可以从小体积的全血样本 (<1 mL) 中执行全自动 LTA 检测，获得高度可重复的结果（变异系数为 3.2%）短时间内（< 25 分钟）。由于反应步骤的自动化、最少的人工操作、稳健的检测策略，使用该设备进行的检测比传统 LTA 产生更精确和准确的结果通过微流体室的独特结构和快速分析，最大限度地减少血小板不稳定的不利影响。

更新日期：2021-11-10

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