Sperm cryopreservation as a routine technique in assisted reproductive technique (ART) laboratories has detrimental effects on spermatozoa. Various methods have been introduced to improve it. The aim of this research was to evaluate the effects of L-proline supplementation in cryopreservation medium on normozoospermic semen samples. A total of 30 semen samples were collected from normozoospermic men. Cryopreservation media were supplemented with different concentrations of L-proline (0, 1, 2 and 4 mmol/L). The semen samples were cryopreserved. After thawing, sperm parameters and chromatin integrity (aniline blue (AB), toluidine blue (TB), sperm chromatin dispersion test (SCD) and chromomycin A3 (CMA3)), reactive oxygen species (ROS), and total antioxidant capacity (TAC) and malondialdehyde (MDA) levels were evaluated. A total of 4 mmol/L L-proline significantly improved progressive motility and viability (p < 0.05). MDA and ROS levels significantly diminished in samples were cryopreserved by 4 mmol/L L-proline supplemented cryopreservation media (p < 0.001). Also, it significantly increased TAC level. Also, chromatin damages (AB, TB and CMA3) significantly improved in samples were cryopreserved by 4 mmol/L L-proline supplemented cryopreservation media (p < 0.05). The results support that the usage of L-proline supplemented cryopreservation media to improve sperm quality after cryopreservation.

中文翻译：

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L-脯氨酸作为冷冻保存培养基的新型添加剂通过降低氧化应激改善人类精子解冻后的质量

精子冷冻保存作为辅助生殖技术 (ART) 实验室的常规技术对精子有不利影响。已经引入了各种方法来改进它。本研究的目的是评估在冷冻保存培养基中添加 L-脯氨酸对正常精子样本的影响。从精子正常的男性中收集了总共 30 份精液样本。冷冻保存培养基中添加了不同浓度的 L-脯氨酸（0、1、2 和 4 mmol/L）。精液样品被冷冻保存。解冻后，精子参数和染色质完整性（苯胺蓝 (AB)、甲苯胺蓝 (TB)、精子染色质分散测试 (SCD) 和色霉素 A3 (CMA3)）、活性氧 (ROS) 和总抗氧化能力 (TAC)和丙二醛（MDA）水平进行了评估。p  < 0.05)。加入 4 mmol/L L-脯氨酸的冻存培养基冻存样品中 MDA 和 ROS 水平显着降低（p  < 0.001）。此外，它显着提高了 TAC 水平。此外，在添加 4 mmol/L L-脯氨酸的冷冻保存培养基中冷冻保存的样品中染色质损伤（AB、TB 和 CMA3）显着改善（p  < 0.05）。结果支持使用L-脯氨酸补充冷冻保存培养基以提高冷冻保存后的精子质量。