半枝莲叶中的生物活性物质及其代谢物通过保护肠道屏障、减轻氧化应激和调节肠道微生物群来改善 DSS 诱导的结肠炎,Food & Function

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半枝莲叶中的生物活性物质及其代谢物通过保护肠道屏障、减轻氧化应激和调节肠道微生物群来改善 DSS 诱导的结肠炎
Food & Function ( IF 5.1 ) Pub Date : 2021-10-12 , DOI: 10.1039/d1fo02588k
Tong Wu ₁ , Xiaoya Wang ₂ , Hua Xiong ₁ , Zeyuan Deng ₁ , Xin Peng ₃ , Lihua Xiao ₁ , Li Jiang ₂ , Yong Sun ₁

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我国名贵药用植物四柱香因其在治疗多种疾病方面具有很高的传统价值，近年来引起了广泛的研究关注。建立体外消化和结肠发酵模型评价半枝莲的稳定性叶 (THL) 酚类的 HPLC-QqQ-MS/MS 方法。在消化和发酵过程中，总酚和类黄酮含量被降解。3-咖啡酰奎尼酸、5-咖啡酰奎尼酸、荭草素和（异）牡荆素被消化酶和肠道微生物群代谢，并以糖苷和较小的酚酸形式被吸收用于肝脏代谢。探讨了THL对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的保护作用及其潜在机制。结果表明，补充THL增加了体重和结肠长度，THL上调了紧密连接蛋白包括occludin、claudin-1和ZO-1的表达水平。含有IL-1β、IL-6和TNF-α的促炎细胞因子的分泌明显受到抑制，而 THL 治疗组抗炎细胞因子 IL-10 的含量增加。此外，THL处理激活了Nrf2的核转移，提高了SOD、CAT、HO-1、NQO1和GCLC的表达，降低了MPO和MDA的含量。值得注意的是，THL 处理显着增加了短链脂肪酸（SCFAs）的含量，增加了Ruminococcaceae，并降低与促炎细胞因子正相关的疣微菌的丰度。这些结果表明，THL通过维持肠上皮屏障有效抑制DSS诱导的结肠炎，通过调节Keap1/Nrf2信号通路减轻氧化应激，调节肠道菌群结构失衡。

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Bioactives and their metabolites from Tetrastigma hemsleyanum leaves ameliorate DSS-induced colitis via protecting the intestinal barrier, mitigating oxidative stress and regulating the gut microbiota

Tetrastigma hemsleyanum, a precious edible and medicinal plant in China, has attracted extensive research attention in recent years due to its high traditional value for the treatment of various diseases. In vitro digestion and colonic fermentation models were established to evaluate the stability of Tetrastigma hemsleyanum leaves (THL) phenolics by the HPLC-QqQ-MS/MS method. The total phenolic and flavonoid contents were degraded during digestion and fermentation. 3-caffeoylquinic acid, 5-caffeoylquinic acid, orientin and (iso)vitexin were metabolized by digestive enzymes and the gut microbiota, and absorbed in the form of glycosides and smaller phenolic acids for hepatic metabolism. The protective effects of THL on dextran sodium sulfate (DSS)-induced colitis in mice and potential mechanisms were explored. The results showed that THL supplementation increased the body weight and colon length, and the expression levels of tight junction proteins including occludin, claudin-1 and ZO-1 were up-regulated by THL. The secretions of pro-inflammatory cytokines containing IL-1β, IL-6 and TNF-α were significantly suppressed, whereas the content of anti-inflammatory cytokine IL-10 was promoted in the THL treated group. In addition, THL treatment activated the nuclear transfer of Nrf2, improved the expression of SOD, CAT, HO-1, NQO1 and GCLC, and decreased the content of MPO and MDA. It is worth noting that THL treatment significantly increased the content of short-chain fatty acids (SCFAs), increased the abundance of Ruminococcaceae, and decreased the abundance of Verrucomicrobia which is positively correlated with pro-inflammatory cytokines. These results indicated that THL effectively inhibited DSS-induced colitis by maintaining the intestinal epithelial barrier, mitigated oxidative stress through regulating the Keap1/Nrf2 signaling pathway and regulated the imbalance of the intestinal flora structure.

更新日期：2021-11-08

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