<br>豌豆蚜 Acyrthosiphon pisum 大脑中隐花色素表达的定位和定量,Insect Molecular Biology

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豌豆蚜 Acyrthosiphon pisum 大脑中隐花色素表达的定位和定量
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2021-11-07 , DOI: 10.1111/imb.12747
Miquel Barberà ₁ , Jorge Mariano Collantes-Alegre ₁ , David Martínez-Torres ₁

Affiliation

蚜虫是典型的光周期动物，常用于研究生物钟在季节反应中的作用。之前，我们描述了生物钟核心（基因period和timeless ）和输出（褪黑素、 AANAT和PTTH ）的一些元素，它们可能在蚜虫季节性反应的调节中发挥作用。最近，我们确定了两种视蛋白（ C-ops和SWO4 ）作为候选输入光周期受体。在本报告中，我们重点研究作为生物钟光感受器的隐花色素（哭泣），并讨论它们在季节性反应中的参与。我们分析了cry1和cry2基因在昼夜节律和季节背景下的表达，并绘制了它们在大脑中的表达位点。我们观察到cry2的强节奏表达在黄昏时达到峰值，与核心时钟基因period和timeless同相，而cry1表现出较弱的节奏。 cry1和cry2表达的变化与季节性反应的激活相关，这表明可能存在联系。最后，我们将cry1和cry2基因的表达映射到外侧部的时钟神经元，外侧部是光周期反应所必需的区域。我们的结果支持cry作为蚜虫生物钟元素的作用，并表明cry1在光接收中和cry2在时钟抑制中发挥作用。

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Mapping and quantification of cryptochrome expression in the brain of the pea aphid Acyrthosiphon pisum

Aphids are paradigmatic photoperiodic animals often used to study the role of the circadian clock in the seasonal response. Previously, we described some elements of the circadian clock core (genes period and timeless) and output (melatonin, AANATs and PTTH) that could have a role in the regulation of the aphid seasonal response. More recently, we identified two opsins (C-ops and SWO4) as candidate input photoperiodic receptors. In the present report, we focus on the study of cryptochromes (cry) as photoreceptors of the circadian clock and discuss their involvement in the seasonal response. We analyse the expression of cry1 and cry2 genes in a circadian and seasonal context, and map their expression sites in the brain. We observe a robust rhythmic expression of cry2 peaking at dusk in phase with core clock genes period and timeless, while cry1 shows a weaker rhythm. Changes in cry1 and cry2 expression correlate with activation of the seasonal response, suggesting a possible link. Finally, we map the expression of cry1 and cry2 genes to clock neurons in the pars lateralis, a region essential for the photoperiodic response. Our results support a role for cry as elements of the aphid circadian clock and suggest a role in photoreception for cry1 and in clock repression for cry2.

更新日期：2021-11-07

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