视网膜模式和神经视蛋白 (Opn5) 视网膜神经节细胞的细胞库,The Journal of Comparative Neurology

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视网膜模式和神经视蛋白 (Opn5) 视网膜神经节细胞的细胞库
The Journal of Comparative Neurology ( IF 2.3 ) Pub Date : 2021-11-07 , DOI: 10.1002/cne.25272
Shane P D'Souza _{1,

2} , David I Swygart ₃ , Sophia R Wienbar ₃ , Brian A Upton _{1,

2,

4} , Kevin X Zhang _{1,

2,

4} , Robert D Mackin ₅ , Anna K Casasent ₅ , Melanie A Samuel ₅ , Gregory W Schwartz _{3,

6} , Richard A Lang _{2,

7,

8}

Affiliation

获取视网膜感觉成分的零件清单对于了解光线对行为、健康和疾病的影响至关重要。视杆细胞、视锥细胞和固有光敏性视网膜神经节细胞 (ipRGC) 是哺乳动物视网膜中描述得最好的光感受器，但最近提出了视网膜神经视蛋白 (Opn5)（一种非典型视蛋白）的功能作用。然而，关于Opn5在视网膜中的表达模式知之甚少。使用 cre ( Opn5 ^cre ) 和 cre 依赖性报告基因，我们发现Opn5表达模式并发现Opn5仅限于视网膜神经节细胞 (RGC)。欧普5-RGC 在视网膜中分布不均匀，位于背颞象限的细胞密度更大。除了这些细胞的局部拓扑结构外，我们使用依赖于 cre 的 AAV 病毒追踪，调查了它们的中心目标，发现它们偏向于图像形成和图像稳定区域。最后，分子和电生理学分析表明Opn5 -RGC 包含先前定义的 RGC 类型，它们对边缘和物体运动做出最佳响应（F-mini-ON、HD2、HD1、LED、ooDSRGC 等）。总之，这些数据描述了在小鼠中表达非典型视蛋白的第二组 RGC，并扩展了成像细胞在视网膜生理和功能中的作用。

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Retinal patterns and the cellular repertoire of neuropsin (Opn5) retinal ganglion cells

Obtaining a parts list of the sensory components of the retina is vital to understand the effects of light in behavior, health, and disease. Rods, cones, and intrinsically photosensitive retinal ganglion cells (ipRGCs) are the best described photoreceptors in the mammalian retina, but recent functional roles have been proposed for retinal neuropsin (Opn5)—an atypical opsin. However, little is known about the pattern of Opn5 expression in the retina. Using cre (Opn5^cre) and cre-dependent reporters, we uncover patterns of Opn5 expression and find that Opn5 is restricted to retinal ganglion cells (RGCs). Opn5-RGCs are nonhomogenously distributed through the retina, with greater densities of cells located in the dorsotemporal quadrant. In addition to the local topology of these cells, using cre-dependent AAV viral tracing, we surveyed their central targets and found that they are biased towards image-forming and image-stabilizing regions. Finally, molecular and electrophysiological profiling reveal that Opn5-RGCs comprise previously defined RGC types that respond optimally to edges and object-motion (F-mini-ONs, HD2, HD1, LEDs, ooDSRGCs, etc.). Together, these data describe the second collection of RGCs that express atypical opsins in the mouse, and expand the roles of image-forming cells in retinal physiology and function.

更新日期：2021-11-07

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