2,5-Bis(hydroxymethyl)furan (BHMF) is one kind of important upgraded derivatives of biobased 5-hydroxymethylfuran (5-HMF). This study verified the feasibility of one-pot chemoenzymatic conversion of biobased D-fructose to 2,5-bis(hydroxymethyl)furan by cascade catalysis with deep eutectic solvent Lactic acid:Betaine (LA:B) and reductase biocatalyst in LA:B−H₂O. Using D-fructose (36.0 g/L) as feedstock, the yield of 5-hydroxymethylfuran reached 91.6% in DES LA:B−H₂O (15:85, v:v) at 150 °C for 1.5 h. Using D-fructose (2 mol D-fructose/mol 5-HMF) as cosubstrate, commercial 5-hydroxymethylfuran (125 mM) was converted into 2,5-bis(hydroxymethyl)furan at 90.7% yield by whole-cells of Pseudomonas putida S12 within 24 h at 30 °C and pH 8.0. In addition, Pseudomonas Putida S12 could efficiently transform D-fructose-valorized 5-hydroxymethylfuran into 2,5-bis(hydroxymethyl)furan [98.4% yield, based on 5-hydroxymethylfuran; 90.1% yield, based on substrate D-fructose] in DES LA:B−H₂O. An efficient chemoenzymatic valorization of D-fructose to 2,5-bis(hydroxymethyl)furan was developed in a benign reaction system.

2,5-双(羟甲基)呋喃(BHMF)是生物基5-羟甲基呋喃(5-HMF)的一种重要升级衍生物。本研究验证了在 LA:B− 中使用低共熔溶剂乳酸:甜菜碱 (LA:B) 和还原酶生物催化剂通过级联催化将生物基D-果糖一锅化学酶促转化为 2,5-双（羟甲基）呋喃的可行性H ₂ O。以D-果糖（36.0 g/L）为原料，在DES LA:B-H ₂ O（15:85，v:v）中，150°C 1.5时，5-羟甲基呋喃的产率达到91.6% H。使用D-果糖（2 mol D-果糖/mol 5-HMF）作为共底物，商业 5-羟甲基呋喃（125 mM）被转化为 2,5-双（羟甲基）呋喃，产率为 90.7%。恶臭假单胞菌S12 在 30 °C 和 pH 8.0 下 24 小时内。此外，Pseudomonas Putida S12 可以有效地将D-果糖转化的5-羟甲基呋喃转化为 2,5-双（羟甲基）呋喃 [98.4% 的产率，基于 5-羟甲基呋喃；90.1% 的产率，基于DES LA:B-H ₂ O 中的底物D-果糖]。在良性反应系统中开发了一种有效的将D-果糖化学酶促转化为 2,5-双（羟甲基）呋喃的方法。