多巴胺与两性离子 DMPC 和阴离子 DMPS 多层囊泡膜的相互作用,Langmuir

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多巴胺与两性离子 DMPC 和阴离子 DMPS 多层囊泡膜的相互作用
Langmuir ( IF 3.7 ) Pub Date : 2021-11-03 , DOI: 10.1021/acs.langmuir.1c02184
Anand Kumar Sahu ₁ , Ashok Kumar Mishra ₁

Affiliation

多巴胺 (DA) 是一种天然存在的神经递质，在哺乳动物神经系统的功能中起着至关重要的作用。在神经传递过程中，DA-脂质-膜相互作用是不可避免的。在本报告中，我们研究了 DA 与阴离子 1,2-dimyristoyl- sn -glycero -3- phospho- l - serine (DMPS)、中性（两性离子）1,2-dimyristoyl- sn的相互作用。-glycero-3-phosphocholine (DMPC) 和突触膜模拟混合 DMPC/DMPS（3:1 摩尔比）模型多层囊泡 (MLV) 膜。差示扫描量热法 (DSC) 研究表明 DA 与阴离子 DMPS 膜具有强烈的特异性相互作用，与两性离子 DMPC 膜的相互作用较弱，与混合 DMPC/DMPS (3:1) 膜的相互作用适中。DA 的固有荧光被用作获得对 DA-脂质-膜相互作用的分子水平理解的新方法。为此，首次对 DA 进行了详细的光物理研究，包括其稳态荧光各向异性和荧光寿命。通过使用固有荧光研究了 DMPS 和 DMPC 模型膜中 DA 的分配系数、位置和分布。还使用 DA 的固有荧光检查了 DA 对模型膜相变的影响。Zeta 电位研究表明 DA 与阴离子 DMPS 膜的强静电相互作用以及 DA 与两性离子 DMPC 膜的非特异性、相对较弱的相互作用。此外，我们观察到胆固醇诱导的 DA 从 DMPS 和 DMPC 膜中排出。我们相信这项工作将在分子水平上提供对 DA-膜相互作用的更深入了解。我们观察到胆固醇诱导的 DA 从 DMPS 和 DMPC 膜中排出。我们相信这项工作将在分子水平上提供对 DA-膜相互作用的更深入了解。我们观察到胆固醇诱导的 DA 从 DMPS 和 DMPC 膜中排出。我们相信这项工作将在分子水平上提供对 DA-膜相互作用的更深入了解。

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Interaction of Dopamine with Zwitterionic DMPC and Anionic DMPS Multilamellar Vesicle Membranes

Dopamine (DA), a naturally occurring neurotransmitter, plays a crucial role in the function of the mammalian nervous system. DA–lipid-membrane interaction is inevitable during the neurotransmission process. In this report, we have studied the interaction of DA with anionic 1,2-dimyristoyl-sn-glycero-3-phospho-l-serine (DMPS), neutral (zwitterionic) 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and synaptic membrane-mimicking mixed DMPC/DMPS (3:1 molar ratio) model multilamellar vesicle (MLV) membranes. Differential scanning calorimetry (DSC) studies suggest a strong specific interaction of DA with the anionic DMPS membrane, a weak interaction with the zwitterionic DMPC membrane, and a moderate interaction with the mixed DMPC/DMPS (3:1) membrane. The intrinsic fluorescence of DA was used as a new approach to gain a molecular-level understanding of DA–lipid-membrane interaction. Toward this end, a detailed photophysical study of DA, including its steady-state fluorescence anisotropy and fluorescence lifetime, was undertaken for the first time. The partition coefficient, location, and distribution of DA in the DMPS and DMPC model membranes were studied by employing intrinsic fluorescence. The effect of DA on the phase transition of the model membranes was also examined using the intrinsic fluorescence of DA. Zeta potential studies suggest a strong electrostatic interaction of DA with the anionic DMPS membrane and a nonspecific, relatively weak interaction of DA with the zwitterionic DMPC membrane. In addition, we observed cholesterol-induced DA expulsion from both DMPS and DMPC membranes. We believe that this work will provide a more in-depth understanding of DA–membrane interaction at a molecular level.

更新日期：2021-11-16

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