用于组织工程的基于硫烯和聚己内酯甲基丙烯酸酯的聚合高内相乳液 (PolyHIPE) 支架,Biomacromolecules

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用于组织工程的基于硫烯和聚己内酯甲基丙烯酸酯的聚合高内相乳液 (PolyHIPE) 支架
Biomacromolecules ( IF 5.5 ) Pub Date : 2021-11-03 , DOI: 10.1021/acs.biomac.1c01129
Betül Aldemir Dikici _{1,

2,

3} , Atra Malayeri ₁ , Colin Sherborne ₁ , Serkan Dikici _{1,

3} , Thomas Paterson ₄ , Lindsey Dew ₁ , Paul Hatton ₄ , Ilida Ortega Asencio ₄ , Sheila MacNeil ₁ , Caitlin Langford ₅ , Neil R Cameron _{5,

6} , Frederik Claeyssens _{1,

2}

Affiliation

高度多孔的乳液模板聚合物 (PolyHIPEs) 在制造用于组织工程和再生医学的支架方面具有许多潜在优势。孔隙率使细胞向内生长和营养物质在支架内扩散，以及从支架中去除废物。乳液模板单独提供的特性包括提供高互连孔隙率，并且与增材制造相结合，可以将受控的多尺度孔隙率引入复杂或定制结构。然而，报告的用于 PolyHIPE 制备的大多数单体系统不适合临床应用，因为它们是不可降解的。硫醇-烯化学是生产可生物降解的光固化PolyHIPEs的有前途的途径，用于使用传统或增材制造方法制造支架；然而，关于这种方法的研究相对较少。这项研究报告了通过光引发的硫醇点击反应制造基于硫醇和聚己内酯 (PCL) 的 PolyHIPE 材料的基础工作。在 PolyHIPE 配方中使用了两种不同的配方，即三臂 PCL 甲基丙烯酸酯 (3PCLMA) 或四臂 PCL 甲基丙烯酸酯 (4PCLMA) 部分。使用人真皮成纤维细胞 (HDF) 和人骨肉瘤细胞系 (MG-63) 通过 DNA 定量分析研究了 PolyHIPE 的生物相容性，并显示开发的 PolyHIPE 能够支持细胞附着和活力。在 PolyHIPE 配方中使用了两种不同的配方，即三臂 PCL 甲基丙烯酸酯 (3PCLMA) 或四臂 PCL 甲基丙烯酸酯 (4PCLMA) 部分。使用人真皮成纤维细胞 (HDF) 和人骨肉瘤细胞系 (MG-63) 通过 DNA 定量分析研究了 PolyHIPE 的生物相容性，并显示开发的 PolyHIPE 能够支持细胞附着和活力。在 PolyHIPE 配方中使用了两种不同的配方，即三臂 PCL 甲基丙烯酸酯 (3PCLMA) 或四臂 PCL 甲基丙烯酸酯 (4PCLMA) 部分。使用人真皮成纤维细胞 (HDF) 和人骨肉瘤细胞系 (MG-63) 通过 DNA 定量分析研究了 PolyHIPE 的生物相容性，并显示开发的 PolyHIPE 能够支持细胞附着和活力。

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Thiolene- and Polycaprolactone Methacrylate-Based Polymerized High Internal Phase Emulsion (PolyHIPE) Scaffolds for Tissue Engineering

Highly porous emulsion templated polymers (PolyHIPEs) provide a number of potential advantages in the fabrication of scaffolds for tissue engineering and regenerative medicine. Porosity enables cell ingrowth and nutrient diffusion within, as well as waste removal from, the scaffold. The properties offered by emulsion templating alone include the provision of high interconnected porosity, and, in combination with additive manufacturing, the opportunity to introduce controlled multiscale porosity to complex or custom structures. However, the majority of monomer systems reported for PolyHIPE preparation are unsuitable for clinical applications as they are nondegradable. Thiol-ene chemistry is a promising route to produce biodegradable photocurable PolyHIPEs for the fabrication of scaffolds using conventional or additive manufacturing methods; however, relatively little research has been reported on this approach. This study reports the groundwork to fabricate thiol- and polycaprolactone (PCL)-based PolyHIPE materials via a photoinitiated thiolene click reaction. Two different formulations, either three-arm PCL methacrylate (3PCLMA) or four-arm PCL methacrylate (4PCLMA) moieties, were used in the PolyHIPE formulation. Biocompatibility of the PolyHIPEs was investigated using human dermal fibroblasts (HDFs) and human osteosarcoma cell line (MG-63) by DNA quantification assay, and developed PolyHIPEs were shown to be capable of supporting cell attachment and viability.

更新日期：2021-11-03

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