Rocking single-use bioreactors (SUBs) adopt an effective mixing mechanism based on the rocking movement of a platform on which a disposable bag-like container is placed. Non-typical liquid flow patterns make the hydrodynamical properties of rocking SUBs not yet well systematized. The aim of the study was to determine a quantitative characteristic of mixing time in a ReadyToProcess WAVE 25 system equipped with a 2-litre Cellbag disposable container, with the use of a sensor method and the Design of Experiment (DoE) methodology. The DoE-based approach has been used to screen significant operational parameters and evaluate their impact on the values of mixing time reached in the studied rocking SUB system. To quantify relations between operational parameters and important process quantities in rocking disposable bag-like containers of different sizes, a set of original formulas for generalization of experimental data and predicting mixing time values has been proposed. The set includes exponential correlations and a correlation involving a dimensionless filling level term and a modified Reynolds number expression. Comparisons between estimations and experimental data have shown satisfactory levels of accuracy within ± 30% of experimental values. The correlation involving dimensionless terms can be applied for disposable bag-like containers offered by different manufacturers and of various sizes.

摇摆式一次性生物反应器 (SUB) 基于放置一次性袋状容器的平台的摇摆运动，采用有效的混合机制。非典型的液体流动模式使得摇摆 SUB 的流体动力学特性尚未很好地系统化。该研究的目的是确定ReadyToProcess中混合时间的定量特征WAVE 25 系统配备 2 升 Cellbag 一次性容器，使用传感器方法和实验设计 (DoE) 方法。基于 DoE 的方法已被用于筛选重要的操作参数并评估它们对所研究的摇摆 SUB 系统中达到的混合时间值的影响。为了量化摇摆不同尺寸的一次性袋状容器的操作参数与重要工艺量之间的关系，提出了一套用于泛化实验数据和预测混合时间值的原始公式。该集合包括指数相关性和涉及无量纲填充水平项和修正雷诺数表达式的相关性。估计值和实验数据之间的比较表明，在实验值的 ± 30% 范围内具有令人满意的准确度水平。涉及无量纲项的相关性可以应用于不同制造商提供的各种尺寸的一次性袋状容器。