反胶束封装作为细胞内拥挤的模型,Journal of the American Chemical Society

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反胶束封装作为细胞内拥挤的模型
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2009-06-17 , DOI: 10.1021/ja901871n
Wade D. Van Horn ₁ , Mark E. Ogilvie ₁ , Peter F. Flynn ₁

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反胶束是由表面活性剂包围的水核组成的离散纳米级颗粒。反胶束核心内的水量可以很容易地控制，以直接影响反胶束颗粒的大小。反胶束的载水能力随温度而变化，如果反胶束暴露在低温下，水会脱落。使用从反胶束中脱落的水可以精确和全面地控制可用于溶剂化主体分子的水量。可以研究封装在反胶束中的蛋白质，以确定限制和排除体积的影响。此处提供的数据在常用的体外稀释研究与体内发现的拥挤环境影响研究之间架起了重要的桥梁。在不同程度的限制下，泛素被封装在双（2-乙基己基）磺基琥珀酸钠 AOT 反胶束中，并与常用分子拥挤剂牛血清白蛋白中的泛素类似重构样品进行比较。使用酰胺 (1) H 和 (15) N 共振的化学位移扰动分析监测封装的影响。结果还协调了反胶束内蛋白质冷变性的替代解释。使用酰胺 (1) H 和 (15) N 共振的化学位移扰动分析监测封装的影响。结果还协调了反胶束内蛋白质冷变性的替代解释。使用酰胺 (1) H 和 (15) N 共振的化学位移扰动分析监测封装的影响。结果还协调了反胶束内蛋白质冷变性的替代解释。

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Reverse Micelle Encapsulation as a Model for Intracellular Crowding

Reverse micelles are discrete nanoscale particles composed of a water core surrounded by surfactant. The amount of water within the core of reverse micelles can be easily manipulated to directly affect the size of the reverse micelle particle. The water loading capacity of reverse micelles varies with temperature, and water can be shed if reverse micelles are exposed to low temperatures. The use of water shedding from the reverse micelle provides precise and comprehensive control over the amount of water available to solvate host molecules. Proteins encapsulated within reverse micelles can be studied to determine the effects of confinement and excluded volume. The data presented here provide an important bridge between commonly employed dilute in vitro studies and studies of the effects of a crowded environment, as found in vivo. Ubiquitin was encapsulated within bis(2-ethylhexyl) sodium sulfosuccinate AOT reverse micelles under various degrees of confinement and was compared with an analogously reconstituted sample of ubiquitin in the commonly used molecular crowding agent bovine serum albumin. The effects of encapsulation were monitored using chemical shift perturbation analysis of the amide (1)H and (15)N resonances. The results also reconcile alternative interpretations of protein cold denaturation within reverse micelles.

更新日期：2009-06-17

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