<br>谷胱甘肽 S-转移酶 (GST) 基因的全基因组分析和 MdGSTU12 的功能鉴定揭示了苹果花青素积累的调控,Genes

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谷胱甘肽 S-转移酶 (GST) 基因的全基因组分析和 MdGSTU12 的功能鉴定揭示了苹果花青素积累的调控
Genes ( IF 2.8 ) Pub Date : 2021-10-29 , DOI: 10.3390/genes12111733
Yu-Wen Zhao ₁ , Chu-Kun Wang ₁ , Xiao-Yu Huang ₁ , Da-Gang Hu ₁

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花青素具有重要的生物学功能，影响着园艺生产的发展。它们通过类黄酮代谢途径在细胞质中合成，最终转运到液泡中储存。植物谷胱甘肽 S-转移酶 (GST) 是参与花青素运输的多功能酶。在本研究中，我们根据与拟南芥GST 家族蛋白的序列相似性，从苹果 ( Malus Domestica ) 基因组 (HFTH1 全基因组 v1.0) 中鉴定了 38 个 GST。这些MdGST基因可分为九个主要亚类：U、F、L、Z、T、GHR、EF1Bγ、TCHQD 和 DHAR。进一步分析了MdGST基因的结构、基序、三维模型和染色体分布。 MdGST的启动子中预测了对某些激素和应激敏感的元素，以及涉及类黄酮生物合成相关基因的其他元素。此外，我们还鉴定了苹果和拟南芥之间的 32 个直系同源基因对。这些基因表明许多苹果和拟南芥对应物似乎源自共同的祖先。在38个MdGST基因中，从报道的表达谱中提取MdGSTU12与花青素变异显着相关。最后，进一步在苹果转基因愈伤组织中的功能鉴定和亚细胞定位证实MdGSTU12在苹果花青素积累中具有重要意义。

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Genome-Wide Analysis of the Glutathione S-Transferase (GST) Genes and Functional Identification of MdGSTU12 Reveals the Involvement in the Regulation of Anthocyanin Accumulation in Apple

Anthocyanins have essential biological functions, affecting the development of horticultural production. They are synthesized in the cytoplasm through flavonoid metabolic pathways and finally transported into vacuoles for storage. Plant glutathione S-transferases (GSTs) are multifunctional enzymes involved in anthocyanin transportation. In this study, we identified 38 GSTs from the apple (Malus domestica) genome (HFTH1 Whole Genome v1.0) based on the sequence similarity with the GST family proteins of Arabidopsis. These MdGST genes could be grouped into nine chief subclasses: U, F, L, Z, T, GHR, EF1Bγ, TCHQD, and DHAR. The structures, motifs, three-dimensional models, and chromosomal distribution of MdGST genes were further analyzed. Elements which are responsive for some hormones and stress, and others that involve genes related to flavonoid biosynthesis were forecast in the promoter of MdGST. In addition, we identified 32 orthologous gene pairs between apple and Arabidopsis. These genes indicated that numerous apple and Arabidopsis counterparts appeared to be derived from a common ancestor. Amongst the 38 MdGST genes, MdGSTU12 was considerably correlated with anthocyanin variation in terms of extracting expression profiles from reported. Finally, further functional identification in apple transgenic calli and subcellular localization confirmed that MdGSTU12 was of great significance in anthocyanin accumulation in apple.

更新日期：2021-10-29

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