Complex cellular environments imaged by SERS nanoprobes using sugars as an all-in-one vector,Journal of Materials Chemistry B

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Complex cellular environments imaged by SERS nanoprobes using sugars as an all-in-one vector
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2021-10-09 , DOI: 10.1039/d1tb01360b
Maria C Gomes _{1,

2} , Juan Chen ₂ , Angela Cunha ₃ , Tito Trindade ₄ , Gang Zheng _{2,

5} , João P C Tomé _{1,

6}

Affiliation

Raman spectroscopy coupled with confocal microscopy offers an alternative bioimaging technique overcoming limitations associated with sensitivity, tissue penetration and image resolution. Allied to the surface-enhanced Raman scattering (SERS) properties of gold nanoparticles (AuNP), we designed SERS nanoprobes with enhanced properties and straightforward application as bio-labelling agents for gliomas. The ensuing nanoprobes coated with simple sugar units (galactose or glucose) allowed assessing information about their intracellular localization (vesicular structures), with impressive sensitivity towards complex environments and proved the ability to overcome biological auto-fluorescence and high penetration in tissues. We validate the use of sugars as an all-in-one vector (Raman reporter, conferring high stability, biocompatibility and affinity to glioma cells) as imaging agents using an impressive technique.

中文翻译：

使用糖作为一体式载体的 SERS 纳米探针成像的复杂细胞环境

拉曼光谱与共聚焦显微镜相结合提供了一种替代生物成像技术，克服了与灵敏度、组织穿透和图像分辨率相关的限制。结合金纳米粒子 (AuNP) 的表面增强拉曼散射 (SERS) 特性，我们设计了具有增强特性的 SERS 纳米探针，可直接用作胶质瘤的生物标记剂。随后涂有单糖单元（半乳糖或葡萄糖）的纳米探针允许评估有关其细胞内定位（囊泡结构）的信息，对复杂环境具有令人印象深刻的敏感性，并证明了克服生物自发荧光和组织中高穿透性的能力。我们验证了糖类作为多合一载体的用途（拉曼报告，赋予高稳定性，

更新日期：2021-10-28

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