Linear Epitopes Play an Important Role in the Immunoglobulin G (IgG)/Immunoglobulin E (IgE)-Binding Capacity of Scy p 4,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Linear Epitopes Play an Important Role in the Immunoglobulin G (IgG)/Immunoglobulin E (IgE)-Binding Capacity of Scy p 4
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-10-24 , DOI: 10.1021/acs.jafc.1c05464
Yi-Yu Chen ₁ , Meng-Si Li ₁ , Meng Liu ₂ , Xiao Yun ₁ , Fei Huan ₁ , Qing-Mei Liu ₁ , Min-Jie Cao ₁ , Gui-Xia Chen ₃ , Dong Lai ₄ , Guang-Ming Liu ₁

Affiliation

Sarcoplasmic calcium-binding protein is a stable allergen in Scylla paramamosain and named Scy p 4. To explore the importance of linear epitopes in the immunoglobulin G (IgG)/immunoglobulin E (IgE)-binding capacity of Scy p 4, chemical denaturants were used to destroy the structure. Scy p 4 was reduced with dithiothreitol and subsequently alkylated with iodoacetamide (IAA). Furthermore, the structural analysis indicated that IAA–Scy p 4 was an unstructured protein. The inhibition enzyme-linked immunosorbent assay showed that IAA–Scy p 4 could inhibit the binding of Scy p 4 to sensitize serum, with inhibition rates reached 55%. Moreover, the linear mimotopes of Scy p 4 were predicted in silico. Three linear epitopes were verified by serological tests and named L-Scy p 4-1 (AA_76–91), L-Scy p 4-2 (AA_111–125), and L-Scy p 4-3 (AA_137–146). Overall, these data provide an understanding of the relationship between the structure and allergenicity about Scy p 4, and the identified linear epitopes can be used for diagnosis and food processing of shellfish allergy.

中文翻译：

线性表位在 Scy p 4 的免疫球蛋白 G (IgG)/免疫球蛋白 E (IgE) 结合能力中发挥重要作用

肌浆钙结合蛋白是Scylla paramamosain 中一种稳定的过敏原，命名为Scy p 4。为了探索线性表位在Scy p 4 的免疫球蛋白 G (IgG)/免疫球蛋白 E (IgE) 结合能力中的重要性，使用化学变性剂来破坏结构。Scy p 4 用二硫苏糖醇还原，随后用碘乙酰胺 (IAA) 烷基化。此外，结构分析表明 IAA-Scy p 4 是一种非结构化蛋白质。抑制酶联免疫吸附试验表明，IAA-Scy p 4 可以抑制Scy p 4 与血清致敏的结合，抑制率达到55%。此外，Scy p 4 的线性拟表位是在计算机中预测的。三个线性表位通过血清学测试验证并命名为 L-Scy p 4-1 (AA_76–91 )、L- _Scy p 4-2 (AA _111–125 ) 和 L- _Scy p 4-3 (AA _137–146 )。总体而言，这些数据提供了对Scy p 4结构和致敏性关系的理解，鉴定的线性表位可用于贝类过敏的诊断和食品加工。

更新日期：2021-11-03

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南