Single Biomolecule Imaging by Electrochemiluminescence,Journal of the American Chemical Society

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Single Biomolecule Imaging by Electrochemiluminescence
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2021-10-22 , DOI: 10.1021/jacs.1c06673
Yujie Liu ₁ , Hongding Zhang ₁ , Binxiao Li ₁ , Jianwei Liu ₁ , Dechen Jiang ₂ , Baohong Liu ₁ , Neso Sojic ₃

Affiliation

Herein, a single biomolecule is imaged by electrochemiluminescence (ECL) using Ru(bpy)₃²⁺-doped silica/Au nanoparticles (RuDSNs/AuNPs) as the ECL nanoemitters. The ECL emission is confined to the local surface of RuDSNs leading to a significant enhancement in the intensity. To prove the concept, a single protein molecule at the electrode is initially visualized using the as-prepared RuDSN/AuNPs nanoemitters. Furthermore, the nanoemitter-labeled antibody is linked at the cellular membrane to image a single membrane protein at one cell, without the interference of current and optical background. The success in single-biomolecule ECL imaging solves the long-lasting task in the ultrasensitive ECL analysis, which should be able to provide more elegant information about the protein in cellular biology.

中文翻译：

电化学发光单生物分子成像

_{在此，使用Ru(bpy) 3}²⁺ -掺杂的二氧化硅/Au纳米颗粒(RuDSNs/AuNPs)作为ECL纳米发射体，通过电化学发光(ECL)对单个生物分子进行成像。ECL 发射仅限于 RuDSN 的局部表面，导致强度显着增强。为了证明这一概念，最初使用准备好的 RuDSN/AuNPs 纳米发射器对电极上的单个蛋白质分子进行可视化。此外，纳米发射体标记的抗体连接在细胞膜上，以在一个细胞上对单个膜蛋白进行成像，而不受电流和光学背景的干扰。单生物分子 ECL 成像的成功解决了超灵敏 ECL 分析中的长期任务，这应该能够提供有关细胞生物学中蛋白质的更优雅的信息。

更新日期：2021-11-03

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