PdeA is required for the rod shape morphology of Brucella abortus,Molecular Microbiology

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PdeA is required for the rod shape morphology of Brucella abortus
Molecular Microbiology ( IF 2.6 ) Pub Date : 2021-10-18 , DOI: 10.1111/mmi.14833
Angéline Reboul ₁ , Elodie Carlier ₁ , François-Xavier Stubbe ₂ , Emeline Barbieux ₁ , Aurore Demars ₁ , Phuong Thi Anh Ong ₁ , Antoine Gerodez ₁ , Eric Muraille _{1,

3} , Xavier De Bolle ₁

Affiliation

Cyclic-di-GMP plays crucial role in the cell cycle regulation of the α-Proteobacterium Caulobacter crescentus. Here we investigated its role in the α-Proteobacterium Brucella abortus, a zoonotic intracellular pathogen. Surprisingly, deletion of all predicted cyclic-di-GMP synthesizing or degrading enzymes did not drastically impair the growth of B. abortus, nor its ability to grow inside cell lines. As other Rhizobiales, B. abortus displays unipolar growth from the new cell pole generated by cell division. We found that the phosphodiesterase PdeA, the ortholog of the essential polar growth factor RgsP of the Rhizobiale Sinorhizobium meliloti, is required for rod shape integrity but is not essential for B. abortus growth. Indeed, the radius of the pole is increased by 31 ± 1.7% in a ΔpdeA mutant, generating a coccoid morphology. A mutation in the cyclic-di-GMP phosphodiesterase catalytic site of PdeA does not generate the coccoid morphology and the ΔpdeA mutant kept the ability to recruit markers of new and old poles. However, the presence of PdeA is required in an intra-nasal mouse model of infection. In conclusion, we propose that PdeA contributes to bacterial morphology and virulence in B. abortus, but it is not crucial for polarity and asymmetric growth.

中文翻译：

流产布鲁氏菌的棒状形态需要 PdeA

Cyclic-di-GMP 在α-Proteobacterium Caulobacter crescentus的细胞周期调节中起着至关重要的作用。在这里，我们研究了它在α-Proteobacterium Brucella abortus中的作用，这是一种人畜共患的细胞内病原体。令人惊讶的是，所有预测的环二 GMP 合成或降解酶的缺失并没有显着损害流产双歧杆菌的生长，也没有显着损害其在细胞系内生长的能力。与其他根瘤菌目一样，B. abortus表现出来自细胞分裂产生的新细胞极的单极生长。我们发现了苜蓿根瘤菌的必需极性生长因子 RgsP 的磷酸二酯酶 PdeA, 是杆状完整性所必需的，但对于流产芽孢杆菌的生长不是必需的。实际上，在ΔpdeA突变体中，极点的半径增加了 31 ± 1.7% ，产生了球状形态。PdeA 的环状二 GMP 磷酸二酯酶催化位点的突变不会产生球状形态，并且 Δ pdeA突变体保持招募新极和旧极标记的能力。然而，在鼻内感染小鼠模型中需要 PdeA 的存在。总之，我们提出 PdeA 有助于B. abortus 中的细菌形态和毒力，但它对极性和不对称生长并不重要。

更新日期：2021-12-20

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