子宫内膜基质细胞蜕膜化分泌途径的深刻结构和功能调整,Traffic

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子宫内膜基质细胞蜕膜化分泌途径的深刻结构和功能调整
Traffic ( IF 3.6 ) Pub Date : 2021-10-14 , DOI: 10.1111/tra.12822
Tiziana Anelli _{1,

2} , Marco Dalla Torre ₁ , Elena Borini ₁ , Elisabetta Mangini ₂ , Adele Ulisse ₂ , Claudia Semino ₂ , Roberto Sitia _{1,

2} , Paola Panina-Bordignon _{1,

3}

Affiliation

某些细胞类型必须扩展其胞吐途径以保证蛋白质分泌的效率和保真度。通过蜕膜人类子宫内膜基质细胞 (EnSC) 提供了一个壮观的案例。在月经周期的黄体中期，黄体酮刺激诱导增殖的 EnSC 分化为专业分泌物，释放对有效胚泡植入至关重要的蛋白质。在这里，我们描述了人类 EnSC 系（叔永生化人类子宫内膜基质细胞 (T-HESC)）分泌途径的结构重排。与原代细胞一样，蜕膜化需要增殖停滞和整个分泌途径的协调扩张，而未检测到未折叠蛋白反应 (UPR) 途径的激活。蜕膜也在没有抗坏血酸的情况下进行，尽管某些折叠不依赖于维生素 C 的蛋白质的分泌受损，但它还是胶原蛋白生物合成的重要辅助因子。然而，即使在这些条件下，也无法检测到明显的 UPR 诱导。形态测量分析表明，胞吐途径不会相对于细胞体积增加。因此，与其他细胞类型不同，在增殖停止后细胞大小的协调增加保证了丰富的生产。

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Profound architectural and functional readjustments of the secretory pathway in decidualization of endometrial stromal cells

Certain cell types must expand their exocytic pathway to guarantee efficiency and fidelity of protein secretion. A spectacular case is offered by decidualizing human endometrial stromal cells (EnSCs). In the midluteal phase of the menstrual cycle, progesterone stimulation induces proliferating EnSCs to differentiate into professional secretors releasing proteins essential for efficient blastocyst implantation. Here, we describe the architectural rearrangements of the secretory pathway of a human EnSC line (TERT-immortalized human endometrial stromal cells (T-HESC)). As in primary cells, decidualization entails proliferation arrest and the coordinated expansion of the entire secretory pathway without detectable activation of unfolded protein response (UPR) pathways. Decidualization proceeds also in the absence of ascorbic acid, an essential cofactor for collagen biogenesis, despite also the secretion of some proteins whose folding does not depend on vitamin C is impaired. However, even in these conditions, no overt UPR induction can be detected. Morphometric analyses reveal that the exocytic pathway does not increase relatively to the volume of the cell. Thus, differently from other cell types, abundant production is guaranteed by a coordinated increase of the cell size following arrest of proliferation.

更新日期：2021-12-21

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