<br>乙烯诱导的 NbMYB4L 参与烟草花叶病毒的抗性,Molecular Plant Pathology

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乙烯诱导的 NbMYB4L 参与烟草花叶病毒的抗性
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-10-11 , DOI: 10.1111/mpp.13139
Tong Zhu ₁ , Xue Zhou ₁ , Jian-Long Zhang ₁ , Wei-Hao Zhang ₁ , Li-Pei Zhang ₂ , Chun-Xiang You ₃ , Paula E Jameson ₄ , Peng-Tao Ma ₁ , Shan-Li Guo ₁

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已知多种 MYB 转录因子在植物抵抗环境胁迫方面发挥重要作用。然而，MYB 参与调节植物烟草花叶病毒 (TMV) 抗性的机制仍不清楚。在这项研究中，我们发现不仅本塞姆氏烟草MYB4样参与对TMV的防御，而且乙烯途径也参与MYB4L介导的抵抗。 NbMYB4L的转录在感染 TMV 的本塞姆氏烟草中上调。 NbMYB4L的沉默导致 TMV 复制增强，而NbMYB4L的过度表达则诱导对 TMV 的显着抗性。 NbMYB4L 的转录在 1-氨基环丙烷甲酸（ACC，乙烯前体）预处理的植物中较高，但当乙烯信号通路在 TMV 感染期间被阻断时， NbMYB4L的转录较低。基因表达分析表明，在乙烯不敏感3样1（EIL1）沉默的植物中， NbMYB4L的转录很大程度上受到抑制。电泳迁移率变动分析和染色质免疫沉淀定量PCR（ChIP-qPCR）实验的结果表明，NbEIL1可以直接结合到NbMYB4L启动子的两个特定区域。此外，荧光素酶测定显示 NbEIL1 显着诱导N中MYB4L启动子的报告活性。本塞米阿纳。这些结果表明，NbEIL1 在本塞姆氏烟草中作为NbMYB4L转录的正调节因子发挥作用，对抗 TMV。总的来说，我们的工作揭示了 EIL1 和 MYB4L 构成了一个连贯的前馈回路，参与本塞姆氏烟草对 TMV 抗性的稳健调节。

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Ethylene-induced NbMYB4L is involved in resistance against tobacco mosaic virus in Nicotiana benthamiana

Several MYB transcription factors are known to play important roles in plant resistance to environmental stressors. However, the mechanism governing the involvement of MYBs in regulating tobacco mosaic virus (TMV) resistance in plants is still unclear. In this study, we found that not only is Nicotiana benthamiana MYB4-like involved in defence against TMV, but also that the ethylene pathway participates in MYB4L-mediated resistance. Transcription of NbMYB4L was up-regulated in N. benthamiana infected with TMV. Silencing of NbMYB4L led to intensified TMV replication, whereas overexpression of NbMYB4L induced significant resistance to TMV. Transcription of NbMYB4L was greater in 1-aminocyclopropanecarboxylic acid (ACC, ethylene precursor)-pretreated plants but lower when the ethylene signalling pathway was blocked during TMV infection. Gene expression analysis showed that the transcription of NbMYB4L was largely suppressed in ETHYLENE INSENSITIVE 3-like 1(EIL1)-silenced plants. The results of electrophoretic mobility shift assay and chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) experiments indicated that NbEIL1 could directly bind to two specific regions of the NbMYB4L promoter. Furthermore, a luciferase assay revealed that NbEIL1 significantly induced the reporter activity of the MYB4L promoter in N. benthamiana. These results point to NbEIL1 functioning as a positive regulator of NbMYB4L transcription in N. benthamiana against TMV. Collectively, our work reveals that EIL1 and MYB4L constitute a coherent feed-forward loop involved in the robust regulation of resistance to TMV in N. benthamiana.

更新日期：2021-12-09

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