RIR1 represses plant immunity by interacting with mitochondrial complex I subunit in rice,Molecular Plant Pathology

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RIR1 represses plant immunity by interacting with mitochondrial complex I subunit in rice
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-10-10 , DOI: 10.1111/mpp.13145
Lin An _{1,

2,

3} , Siyuan Zhang _{1,

2,

3} , Ping Guo _{1,

2,

3} , Liyang Song _{1,

2,

3} , Chuanmiao Xie _{1,

2} , Hongyan Guo _{1,

2,

4} , Rongxiang Fang _{1,

2} , Yantao Jia _{1,

2}

Affiliation

We previously observed decreased expression of rice OsmiR159a.1 on infection with the bacterial blight-causing pathogen Xanthomonas oryzae pv. oryzae (Xoo), and identified the OsLRR_RLK (leucine-rich repeat_ receptor like kinase) gene as an authentic target of OsmiR159a.1. Here, we found that a Tos17 insertion mutant of LRR_RLK displayed increasing temporal resistance to Xoo, whereas the LRR_RLK overexpression lines were susceptible to the pathogen early on in the infection, indicating that LRR_RLK encodes a repressor of rice resistance to Xoo infection, and it was renamed as RIR1 (Rice Immunity Repressor 1). RIR1 overexpression plants were more susceptible to Xoo at late growth stage, suggesting that RIR1 mRNA levels are negatively correlated with the resistance of rice against Xoo. We discovered that OsmiR159a.1 repression in Xoo-infected plants was largely dependent on the pathogen's type III secretion system. Co-immunoprecipitation, bimolecular fluoresence complementation, and pull-down assays indicated that RIR1 interacted with the NADH-ubiquinone oxidoreductase (NUO) 51-kDa subunit of the mitochondrial complex I through its kinase domain. Notably, impairment of RIR1 or overexpression of NUO resulted in reactive oxygen species accumulation and enhanced expression of pathogen-resistance genes, including jasmonic acid pathway genes. We propose that pathogens may inhibit OsmiR159 to interfere with the RIR1–NUO interaction, and subsequently depression of rice immune signalling pathways. The resistance genes manipulated by Xoo can be a probe to explore the regulatory network during host–pathogen interactions.

中文翻译：

RIR1通过与水稻线粒体复合物I亚基相互作用抑制植物免疫

我们之前观察到水稻 OsmiR159a.1 在感染引起细菌性枯萎病的病原体Xanthomonas oryzae pv 时表达降低。oryzae (Xoo)，并确定OsLRR_RLK（富含亮氨酸重复受体样激酶）基因是 OsmiR159a.1 的真实靶标。在这里，我们发现LRR_RLK的Tos17插入突变体显示出对 Xoo 的时间抗性增加，而LRR_RLK过表达系在感染早期对病原体敏感，表明LRR_RLK编码水稻对 Xoo 感染的抗性抑制因子，并且它是重命名为RIR1（Rice Immunity Repressor 1）。RIR1过表达植物在生长后期对 Xoo 更敏感，表明RIR1 mRNA 水平与水稻对 Xoo 的抗性呈负相关。我们发现 Xoo 感染植物中 OsmiR159a.1 的抑制很大程度上取决于病原体的 III 型分泌系统。共免疫沉淀、双分子荧光互补和下拉分析表明，RIR1 通过其激酶结构域与线粒体复合物 I 的 NADH-泛醌氧化还原酶 (NUO) 51-kDa 亚基相互作用。值得注意的是，RIR1受损或NUO过表达导致活性氧积累和病原体抗性基因的表达增强，包括茉莉酸途径基因。我们提出病原体可能会抑制 OsmiR159 以干扰 RIR1-NUO 相互作用，从而抑制水稻免疫信号通路。Xoo 操纵的抗性基因可以作为探索宿主-病原体相互作用过程中调控网络的探针。

更新日期：2021-12-09

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