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Duplex-Specific Nuclease-Triggered Fluorescence Immunoassay Based on Dual-Functionalized AuNP for Acetochlor, Metolachlor, and Propisochlor
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-10-08 , DOI: 10.1021/acs.analchem.1c02736 Yonghong Zha 1 , Yansong Li 1 , Pan Hu 1 , Shiying Lu 1 , Honglin Ren 1 , Zengshan Liu 1 , Hualin Yang 2 , Yu Zhou 1, 3
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-10-08 , DOI: 10.1021/acs.analchem.1c02736 Yonghong Zha 1 , Yansong Li 1 , Pan Hu 1 , Shiying Lu 1 , Honglin Ren 1 , Zengshan Liu 1 , Hualin Yang 2 , Yu Zhou 1, 3
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Given the great harm of pesticide residues to the environment and public health, exploring ultrasensitive and low-cost methods for their quantitative analysis becomes intensely necessary. Herein, we proposed a double-functionalized gold nanoparticle (AuNP) probe as a signal amplification immunoassay for the detection of acetochlor (ATC), metolachlor, and propisochlor. The AuNP was modified with IgG and fluorophore-labeled duplex DNA by a polyadenine-based freezing method. The quenched fluorescence can be effectively recovered via duplex-specific nuclease (DSN) with excellent cleaving activity. This approach provided limits of detection (LODs) down to 0.03 ng/mL for ATC, 0.10 ng/mL for metolachlor, 0.14 ng/mL for propisochlor, and 0.08 ng/mL for their mixture. The average recoveries of ATC, metolachlor, and propisochlor were 93.0–106.6% from a corn sample, which are in good agreement with the commercial kit (R2 = 0.9995). This “turn-off” fluorescence immunoassay presents considerable potential in the analysis of chloroacetamide herbicide due to its simple process of probe preparing and ultrahigh sensitivity.
中文翻译：
基于双功能化 AuNP 对乙草胺、异丙甲草胺和异丙草胺进行双链特异性核酸酶触发荧光免疫分析
鉴于农药残留对环境和公众健康的巨大危害，探索超灵敏、低成本的定量分析方法变得非常必要。在此，我们提出了一种双功能化金纳米颗粒 (AuNP) 探针作为信号放大免疫测定法，用于检测乙草胺 (ATC)、异丙甲草胺和丙草胺。通过基于聚腺嘌呤的冷冻方法，用 IgG 和荧光团标记的双链 DNA 修饰 AuNP。猝灭的荧光可以通过具有优异切割活性的双链特异性核酸酶 (DSN) 有效恢复。这种方法提供的检测限 (LOD) 低至 ATC 0.03 ng/mL、异丙甲草胺 0.10 ng/mL、异丙草胺 0.14 ng/mL 和它们混合物的 0.08 ng/mL。ATC、异丙甲草胺和丙草胺的平均回收率为 93.0–106。R 2 = 0.9995）。由于其探针制备过程简单且灵敏度极高，这种“关闭”荧光免疫分析在氯乙酰胺除草剂的分析中具有相当大的潜力。
更新日期：2021-10-19
中文翻译：
基于双功能化 AuNP 对乙草胺、异丙甲草胺和异丙草胺进行双链特异性核酸酶触发荧光免疫分析
鉴于农药残留对环境和公众健康的巨大危害，探索超灵敏、低成本的定量分析方法变得非常必要。在此，我们提出了一种双功能化金纳米颗粒 (AuNP) 探针作为信号放大免疫测定法，用于检测乙草胺 (ATC)、异丙甲草胺和丙草胺。通过基于聚腺嘌呤的冷冻方法，用 IgG 和荧光团标记的双链 DNA 修饰 AuNP。猝灭的荧光可以通过具有优异切割活性的双链特异性核酸酶 (DSN) 有效恢复。这种方法提供的检测限 (LOD) 低至 ATC 0.03 ng/mL、异丙甲草胺 0.10 ng/mL、异丙草胺 0.14 ng/mL 和它们混合物的 0.08 ng/mL。ATC、异丙甲草胺和丙草胺的平均回收率为 93.0–106。R 2 = 0.9995）。由于其探针制备过程简单且灵敏度极高，这种“关闭”荧光免疫分析在氯乙酰胺除草剂的分析中具有相当大的潜力。
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