Nanopipette-Based Nanosensor for Label-Free Electrochemical Monitoring of Cell Membrane Rupture under H2O2 Treatment,Analytical Chemistry

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Nanopipette-Based Nanosensor for Label-Free Electrochemical Monitoring of Cell Membrane Rupture under H2O2 Treatment
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-10-08 , DOI: 10.1021/acs.analchem.1c03313
Xiao-Yuan Wang ₁ , Jian Lv ₁ , Qin Hong ₁ , Ze-Rui Zhou ₁ , Da-Wei Li ₁ , Ruo-Can Qian ₁

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H₂O₂ is an essential signaling molecule in living cells that can cause direct damage to lipids, proteins, and DNA, resulting in cell membrane rupture. However, current studies mostly focus on probe-based sensing of intracellular H₂O₂, and these methods usually require sophisticated probe synthesis and instruments. In particular, local H₂O₂ treatment induces cell membrane rupture, but the level of cell membrane destruction is unknown because the mechanical properties of the cell membrane are difficult to accurately determine. Therefore, highly sensitive and label-free methods are required to measure and reflect mechanical changes in the cell membrane. Here, using an ultrasmall quartz nanopipette with a tip diameter less than 90 nm as a nanosensor, label-free and noninvasive electrochemical single-cell measurement is achieved for real-time monitoring of cell membrane rupture under H₂O₂ treatment. By spatially controlling the nanopipette tip to precisely approach a specific location on the membrane of a single living cell, stable cyclic membrane oscillations are observed under a constant direct current voltage. Specifically, upon nanopipette advancement, the mechanical status of the cell membrane can be sensibly displayed by continuous current versus time traces. The electrical signals are collected and processed, ultimately revealing the mechanical properties of the cell membrane and the degree of cell apoptosis. This nanopipette-based nanosensor paves the way for developing a facile, label-free, and noninvasive strategy to assay the mechanical properties of the cell membrane during external stimulation at the single-cell level.

中文翻译：

基于纳米移液管的纳米传感器用于 H2O2 处理下细胞膜破裂的无标记电化学监测

H ₂ O ₂是活细胞中必不可少的信号分子，可直接损伤脂质、蛋白质和DNA，导致细胞膜破裂。然而，目前的研究主要集中在基于探针的细胞内 H ₂ O ₂传感上，这些方法通常需要复杂的探针合成和仪器。特别是局部 H ₂ O ₂处理会导致细胞膜破裂，但细胞膜破坏的程度尚不清楚，因为细胞膜的机械特性难以准确确定。因此，需要高度灵敏和无标记的方法来测量和反映细胞膜的机械变化。在这里，使用尖端直径小于 90 nm 的超小型石英纳米移液管作为纳米传感器，实现了无标记和无创电化学单细胞测量，用于在 H ₂ O ₂下实时监测细胞膜破裂治疗。通过空间控制纳米吸管尖端精确接近单个活细胞膜上的特定位置，在恒定直流电压下观察到稳定的循环膜振荡。具体而言，随着纳米移液管的进步，细胞膜的机械状态可以通过连续电流与时间的轨迹进行敏感显示。电信号被收集和处理，最终揭示细胞膜的机械特性和细胞凋亡的程度。这种基于纳米移液管的纳米传感器为开发一种简便、无标记和非侵入性的策略铺平了道路，以在单细胞水平的外部刺激期间测定细胞膜的机械特性。

更新日期：2021-10-19

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