Two-photon ESIPT-based fluorescent probe using 4-hydroxyisoindoline-1,3-dione for the detection of peroxynitrite,Chemical Communications

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Two-photon ESIPT-based fluorescent probe using 4-hydroxyisoindoline-1,3-dione for the detection of peroxynitrite
Chemical Communications ( IF 4.3 ) Pub Date : 2021-09-17 , DOI: 10.1039/d1cc03160k
Luling Wu ₁ , Xue Tian ₁ , Dong Joon Lee ₂ , Juyoung Yoon ₃ , Chang Su Lim ₂ , Hwan Myung Kim ₂ , Tony D James _{1,

4}

Affiliation

Excited-state intramolecular proton transfer (ESIPT)-based fluorophores with two-photon excitation fluorescence (TPEF) are rare. Our aim with this research was to develop ESIPT-based fluorophores exhibiting TPEF. Herein, we used 4-hydroxyisoindoline-1,3-dione as a scaffold to develop a two-photon fluorescent probe BHID-Bpin, for the detection of peroxynitrite (ONOO⁻). BHID-Bpin exhibits excellent selectivity, sensitivity, and fast response towards ONOO⁻ in PBS buffer solution (10 mM, pH = 7.40). Additionally, BHID-Bpin displays high photo-stability under two-photon irradiation at 750 nm. Furthermore, the probe can image endogenous ONOO⁻ in HeLa cells and exogenous ONOO⁻ in rat hippocampal slices at a depth of 110 μm.

中文翻译：

基于双光子 ESIPT 的荧光探针，使用 4-羟基异吲哚啉-1,3-二酮检测过氧亚硝酸盐

具有双光子激发荧光 (TPEF) 的基于激发态分子内质子转移 (ESIPT) 的荧光团很少见。我们这项研究的目的是开发具有 TPEF 的基于 ESIPT 的荧光团。在此，我们使用 4-羟基异吲哚啉-1,3-二酮作为支架开发了双光子荧光探针 BHID-Bpin，用于检测过氧亚硝酸盐 (ONOO ^- )。BHID-Bpin在 PBS 缓冲溶液（10 mM，pH = 7.40）中对 ONOO ^-表现出出色的选择性、灵敏度和快速响应。此外，BHID-Bpin 在 750 nm 的双光子照射下显示出高光稳定性。此外，探针可内源性图像ONOO ^-在HeLa细胞和外源性ONOO ^-在大鼠海马切片在110微米的深度。

更新日期：2021-10-07

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