Electrospun polylactic acid/sulfadiazine sodium/proteinase nanofibers and their applications in treating frostbite,Journal of Applied Polymer Science

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Electrospun polylactic acid/sulfadiazine sodium/proteinase nanofibers and their applications in treating frostbite
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2021-10-03 , DOI: 10.1002/app.51716
Chengbiao Ding _{1,

2} , Chenxu Zhou ₁ , Yueyao Fan ₂ , Qi Liu ₂ , Haifeng Zhang ₂ , Zhengwei Wu _{2,

3}

Affiliation

In this study, polylactic acid (PLA)/sulfadiazine sodium/proteinase K composite nanofibers were prepared by electrospinning, and their morphology was observed by scanning electron microscope. The novel nanofibers were characterized by Fourier transform infrared spectroscopy and drug release curve. The activity of different groups of drugs loaded nanofibers in frostbite wound of rats was studied and compared with the blank control group. Hematoxylin–eosin staining and transmission electron microscope were used to access the histological changes of frostbite wounds on the back of rats before and after treatment. CD31 fluorescence staining was used to track the reconstruction and improvement of blood vessels in tissues. The results revealed that the drug release rate of PLA/sulfadiazine sodium/proteinase K nanofibers group was more than 50% at 1 h, and the cumulative drug release rate was more than 88% at 168 h, and CD31 fluorescence showed that its vascular density was 13.27% ± 0.9%, which was significantly higher than that of other groups. Therefore, PLA/sulfadiazine sodium/proteinase K composite nanofibers could promote the healing of frostbite wounds, which indicated the increase of capillaries and the regeneration of hair follicles. PLA/sulfadiazine sodium/proteinase K composite nanofibers have great potential in the application of frostbite.

中文翻译：

电纺聚乳酸/磺胺嘧啶钠/蛋白酶纳米纤维及其在治疗冻伤中的应用

本研究采用静电纺丝法制备聚乳酸（PLA）/磺胺嘧啶钠/蛋白酶K复合纳米纤维，并通过扫描电镜观察其形貌。通过傅里叶变换红外光谱和药物释放曲线对新型纳米纤维进行了表征。研究了载药纳米纤维不同组对大鼠冻伤创面的活性，并与空白对照组进行比较。采用苏木精-伊红染色和透射电镜观察治疗前后大鼠背部冻伤创面的组织学变化。CD31 荧光染色用于跟踪组织中血管的重建和改善。结果表明，PLA/磺胺嘧啶钠/蛋白酶K纳米纤维组的药物释放率在1h时大于50%，168 h累积释药率88%以上，CD31荧光显示其血管密度为13.27%±0.9%，明显高于其他组。因此，PLA/磺胺嘧啶钠/蛋白酶K复合纳米纤维可以促进冻伤创面的愈合，表明毛细血管增多，毛囊再生。PLA/磺胺嘧啶钠/蛋白酶K复合纳米纤维在冻伤方面的应用潜力巨大。这表明毛细血管的增加和毛囊的再生。PLA/磺胺嘧啶钠/蛋白酶K复合纳米纤维在冻伤方面的应用潜力巨大。这表明毛细血管的增加和毛囊的再生。PLA/磺胺嘧啶钠/蛋白酶K复合纳米纤维在冻伤方面的应用潜力巨大。

更新日期：2021-12-01

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