Corneal transplantation is an effective treatment for corneal blindness. However, it brings risk factors for the occurrence of bacterial keratitis, which can affect the repair effect and even lead to transplantation failure. The difficulty in re-epithelialization is also a main problem faced by corneal transplantation. Herein, a collagen-GelMA composite membrane containing lysozyme (CGL) was developed as an antibacterial corneal implant to fill stromal defect and support re-epithelialization. Characterizations of physicochemical properties and in vitro biocompatibility revealed that the composite membranes have proper water content, light transmittance and mechanical strength as well as good biocompatibility. Particularly, the cell adhesion force and adhesion-related genes expression were evaluated and exhibited an improvement after the addition of GelMA. Furthermore, the formed CGL membrane could continuously release lysozyme and exhibited a bactericidal rate of 96% and 64% after 2 h and 72 h, respectively. The results demonstrated that this CGL membrane has promising application in corneal repair.

角膜移植是治疗角膜失明的有效方法。但它为细菌性角膜炎的发生带来了危险因素，会影响修复效果，甚至导致移植失败。再上皮化的困难也是角膜移植面临的主要问题。在此，开发了一种含有溶菌酶 (CGL) 的胶原-GelMA 复合膜作为抗菌角膜植入物，以填充基质缺损并支持再上皮化。物理化学性质和体外表征生物相容性表明复合膜具有适当的含水量、透光率和机械强度以及良好的生物相容性。特别是，在加入 GelMA 后，评估了细胞粘附力和粘附相关基因的表达，并表现出改善。此外，形成的 CGL 膜可以持续释放溶菌酶，并在 2 小时和 72 小时后分别表现出 96% 和 64% 的杀菌率。结果表明，这种 CGL 膜在角膜修复中具有广阔的应用前景。