Atomically Thin Bilayer Janus Membranes for Cryo-electron Microscopy,ACS Nano

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Atomically Thin Bilayer Janus Membranes for Cryo-electron Microscopy
ACS Nano ( IF 15.8 ) Pub Date : 2021-09-27 , DOI: 10.1021/acsnano.1c06233
Liming Zheng ₁ , Nan Liu ₂ , Ying Liu _{3,

4} , Ning Li _{5,

6} , Jincan Zhang _{1,

6} , Chongzhen Wang _{1,

7} , Wenqing Zhu _{3,

4} , Yanan Chen _{2,

8} , Dongchen Ying ₁ , Jie Xu _{2,

9} , Zi Yang _{2,

9} , Xiaoyin Gao ₁ , Jilin Tang _{1,

6} , Xiaoge Wang ₁ , Zibin Liang ₁₀ , Ruqiang Zou ₁₀ , Yuzhang Li ₇ , Peng Gao _{5,

11} , Xiaoding Wei _{3,

4} , Hong-Wei Wang _{2,

9,

12} , Hailin Peng _{1,

6,

13}

Affiliation

Center for Nanochemistry, Beijing Science and Engineering Center for Nanocarbons, Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Ministry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
State Key Laboratory for Turbulence and Complex System, Department of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing 100871, China.
Beijing Innovation Center for Engineering Science and Advanced Technology, Peking University, Beijing 100871, China.
International Center for Quantum Materials and Electron Microscopy Laboratory, School of Physics, Peking University, Beijing 100871, China.
Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles California 90095, United States.
School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing 100084, China.
Beijing Key Laboratory for Theory and Technology of Advanced Battery Materials, School of Materials Science and Engineering, Peking University, Beijing 100871, China.
Collaborative Innovation Center of Quantum Matter, Beijing 100871, China.
Beijing Frontier Research Center for Biological Structures, Tsinghua University, Beijing 100084, China.
Beijing Graphene Institute (BGI), Beijing 100095, China.

Cryo-electron microscopy (cryo-EM) has emerged as a vital tool to reveal the native structure of beam-sensitive biomolecules and materials. Yet high-resolution cryo-EM analysis is still limited by the poorly controlled specimen preparation and urgently demands a robust supporting film material to prepare desirable samples. Here, we developed a bilayer Janus graphene membrane with the top-layer graphene being functionalized to interact with target molecules on the surface, while the bottom layer being kept intact to reinforce its mechanical steadiness. The ultraclean and atomically thin bilayer Janus membrane prepared by our protocol on one hand generates almost no extra noise and on the other hand reduces the specimen motion during cryo-EM imaging, thus allowing the atomic-resolution characterization of surface functional groups. Using such Janus membranes in cryo-EM specimen preparation, we were able to directly image the lithium dendrite and reconstruct macromolecules at near-atomic resolution. Our results demonstrate the bilayer Janus design as a promising supporting material for high-resolution cryo-EM and EM imaging.

中文翻译：

用于冷冻电子显微镜的原子级薄双层 Janus 膜

冷冻电子显微镜 (cryo-EM) 已成为揭示光束敏感生物分子和材料的天然结构的重要工具。然而，高分辨率冷冻电镜分析仍然受到样本制备控制不善的限制，迫切需要一种坚固的支撑膜材料来制备理想的样本。在这里，我们开发了双层 Janus 石墨烯膜，顶层石墨烯被功能化以与表面上的目标分子相互作用，而底层保持完整以增强其机械稳定性。我们的协议制备的超洁净和原子级薄双层 Janus 膜一方面几乎不会产生额外的噪音，另一方面减少了冷冻 EM 成像过程中的样品运动，从而实现了表面官能团的原子分辨率表征。在冷冻电镜样品制备中使用这种 Janus 膜，我们能够直接成像锂枝晶并以近原子分辨率重建大分子。我们的结果表明双层 Janus 设计是高分辨率冷冻 EM 和 EM 成像的有前途的支持材料。

更新日期：2021-10-26

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