<br>棕榈酸和二十碳五烯酸对猪血管内皮细胞血管生成的影响,Veterinary Medicine and Science

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棕榈酸和二十碳五烯酸对猪血管内皮细胞血管生成的影响
Veterinary Medicine and Science ( IF 1.8 ) Pub Date : 2021-09-21 , DOI: 10.1002/vms3.577
Jie Peng _{1,

2} , Menglin Yang _{1,

2} , Guoli Li ₃ , Xiu Zhang ₄ , Yanhua Huang _{1,

2,

3} , Yimei Tang _{1,

5}

Affiliation

胎盘血管生成受限是仔猪宫内生长迟缓的重要原因。妊娠期间，母猪肥胖会导致胎盘脂质沉积增加，从而抑制胎盘血管生成和胎儿发育。然而，不同类型的脂肪酸对胎盘血管生成的影响仍不清楚。滋养层细胞和血管内皮细胞构成胎盘组织的两种重要类型。在本研究中，我们分别使用棕榈酸（C16：0）和二十碳五烯酸（C20：5， n -3）处理猪滋养外胚层细胞（pTr2）和猪髂动脉内皮细胞（PIEC），以研究饱和脂肪酸和n -3 多不饱和脂肪酸（PUFA）对体外胎盘血管生成的影响。我们发现 C16:0 在 pTr2 和 PIEC 中引起显着的细胞毒性 ( p < 0.01)，并抑制 PIEC 的增殖和迁移 ( p < 0.01)，而 C20:5 处理表现出非常低的细胞毒性和对细胞增殖的最小抑制。同时，低浓度的C16:0对PIEC的管形成没有影响，而C20:5显着促进PIEC的管形成( p < 0.01)。这些结果表明饱和脂肪酸和n -3 PUFA对胎盘血管生成有不同的影响。 n -3 PUFA作为必需功能脂肪酸，可能是减轻妊娠期母猪肥胖引起的胎盘脂毒性的有效措施。

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Effects of palmitic acid and eicosapentaenoic acid on angiogenesis of porcine vascular endothelial cells

Restricted placental angiogenesis is an important cause of intrauterine growth retardation in piglets. During pregnancy, sow obesity can result in an increase in placental lipid deposition, subsequently inhibiting placental angiogenesis and fetal development. However, the effect of different types of fatty acids on placental angiogenesis is still unclear. Trophoblast cells and vascular endothelial cells constitute two important types of placental tissue. In this study, we used palmitic acid (C16:0) and eicosapentaenoic acid (C20:5, n-3), respectively, to treat porcine trophectoderm cells (pTr2) and porcine iliac artery endothelial cells (PIEC) to study the effects of saturated fatty acids and n-3 polyunsaturated fatty acids (PUFAs) on placental angiogenesis in vitro. We found that C16:0 caused significant cytotoxicity in pTr2 and PIEC (p < 0.01) and inhibited the proliferation and migration of PIEC (p < 0.01), whereas C20:5 treatment exhibited very low cytotoxicity and minimal inhibition of cellular proliferation. Meanwhile, a low concentration of C16:0 had no effect on the tube formation in PIEC, whereas C20:5 significantly promoted tube formation of PIEC (p < 0.01). These results suggested that saturated fatty acids and n-3 PUFAs had different effects on placental angiogenesis. As essential functional fatty acid, n-3 PUFA might be effective measure in alleviating the placental lipotoxicity caused by sow obesity during pregnancy.

更新日期：2021-11-20

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