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Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway
Journal of Ethnopharmacology ( IF 4.8 ) Pub Date : 2021-09-20 , DOI: 10.1016/j.jep.2021.114664
Rongyao Liang 1 , Xiyang Tong 1 , Zhikui Dong 2 , Weiwei Qin 1 , Lingling Fan 1 , Ziyu Bai 1 , Zhihao Zhang 1 , Ting Xiang 3 , Zhen Wang 1 , Ninghua Tan 1
Affiliation  

Ethnopharmacological relevance

Suhuang antitussive capsule (SH capsule), a typical traditional Chinese medicines (TCMs) compound, is widely used for the treatment of post-infectious cough (PIC) in the clinic. Our previous studies have demonstrated that SH capsule possesses significant ameliorative effects on cough variant asthma (CVA), sputum obstruction and airway remodeling.

Aim of the study

This study is designed to investigate the ameliorative effects and potential mechanisms of SH capsule on PIC in mice.

Materials and methods

To establish the PIC model, ICR mice were induced by lipopolysaccharide (LPS) (3 mg/kg) once, followed by cigarettes smoke (CS) exposure for 30 min per day for 30 days. Mice were intragastrically (i.g.) administrated with SH capsule at the doses of 3.5, 7, 14 g/kg each day for 2 weeks since the 24th day. The number of coughs, coughs latencies, enzyme-linked immunosorbent assay (ELISA) and histological analysis were used to investigate the effects of SH capsule on PIC mice. Quantitative-polymerase chain reaction (Q-PCR) and western blotting were conducted to evaluate the levels of mRNA and proteins associated with Aryl hydrocarbon receptor (AhR)–NF–E2-related factor 2 (Nrf2) pathway. Superoxide dismutase (SOD), glutathione (GSH) and total antioxidant capacity (T-AOC) assays were performed to evaluate the oxidative stress. A549 cells were used to investigate the ameliorative effects of SH capsule in vitro.

Results

SH capsule effectively diminished the number of coughs and extended coughs latencies in PIC mice. The airway inflammation was alleviated by decreasing the expression levels of inflammatory mediators including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6). Moreover, SH capsule dose-dependently activated AhR-Nrf2 pathway and induced the nuclear translocation in vitro and in vivo. Besides, SH capsule significantly increased the levels of SOD, GSH and T-AOC in mice.

Conclusion

Our study demonstrates that SH capsule ameliorates airway inflammation-associated PIC in mice through activating AhR-Nrf2 pathway and consequently alleviating inflammatory responses and oxidative stress.



中文翻译:

苏黄镇咳胶囊通过AhR-Nrf2通路改善小鼠感染后咳嗽

民族药理学相关性

苏黄镇咳胶囊(SH胶囊)是一种典型的中药复方,临床上广泛用于治疗感染后咳嗽(PIC)。我们之前的研究表明,SH胶囊对咳嗽变异性哮喘(CVA)、痰液阻塞和气道重塑具有显着的改善作用。

研究目的

本研究旨在探讨SH胶囊对小鼠PIC的改善作用和潜在机制。

材料和方法

为了建立 PIC 模型,ICR 小鼠被脂多糖 (LPS) (3 mg/kg) 诱导一次,然后每天 30 分钟暴露于香烟烟雾 (CS) 中,持续 30 天。小鼠被灌胃(ig) 从第 24 天开始,以每天 3.5、7、14 g/kg 的剂量与 SH 胶囊一起给药,持续 2 周。通过咳嗽次数、咳嗽潜伏期、酶联免疫吸附试验(ELISA)和组织学分析来研究SH胶囊对PIC小鼠的影响。进行定量聚合酶链反应(Q-PCR)和蛋白质印迹以评估与芳烃受体(AhR)-NF-E2相关因子2(Nrf2)途径相关的mRNA和蛋白质的水平。进行超氧化物歧化酶 (SOD)、谷胱甘肽 (GSH) 和总抗氧化能力 (T-AOC) 测定以评估氧化应激。用A549细胞体外研究SH胶囊的改善作用。

结果

SH 胶囊有效地减少了 PIC 小鼠的咳嗽次数并延长了咳嗽潜伏期。通过降低包括肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素6(IL-6)在内的炎症介质的表达水平来缓解气道炎症。此外,SH胶囊剂量依赖性地激活AhR-Nrf2通路并在体外体内诱导核转位。此外,SH胶囊显着增加了小鼠SOD、GSH和T-AOC的水平。

结论

我们的研究表明,SH 胶囊通过激活 AhR-Nrf2 通路改善小鼠气道炎症相关的 PIC,从而减轻炎症反应和氧化应激。

更新日期:2021-09-30
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