Enhanced biological phosphorus removal (EBPR) is often applied by wastewater treatment plants (WWTPs) whereby phosphorus (P) is accumulated in the activated sludge (AS) biomass. The German Sewage Sludge Ordinance demands that large WWTPs recycle P from their process streams by 2029. This study aimed at evaluating the potential of an acetate-mediated P re-dissolution from non-acclimated AS of three full-scale WWTPs. All AS exhibited enhanced P re-dissolution during acetate uptake and followed kinetics typical of EBPR systems. Re-dissolution efficiency differed depending on the WWTP operation mode. The highest P re-dissolution yield (56% of total P) was obtained for pure EBPR sludge. Lower re-dissolution rates of 19-22% were observed in the AS from WWTPs that employed additional chemical P removal, probably due to shortage of intracellular polyphosphate. Pure EBPR systems may be suited for the implementation of a re-dissolution based strategy for P recycling from AS where P depleted AS could potentially be returned to the biological treatment stage.