七叶苷对白斑综合征病毒的抗病毒活性：虾苗养殖白斑病疫情防控新起点,Journal of Fish Diseases

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七叶苷对白斑综合征病毒的抗病毒活性：虾苗养殖白斑病疫情防控新起点
Journal of Fish Diseases ( IF 2.2 ) Pub Date : 2021-09-18 , DOI: 10.1111/jfd.13533
Li-Peng Shan _{1,

2,

3} , Xu Zhang _{1,

2,

3} , Yang Hu _{1,

2,

3} , Lei Liu _{1,

2,

3} , Jiong Chen _{1,

2,

3}

Affiliation

白斑综合征病毒（WSSV）是一种对虾养殖中的致病性和威胁性病毒，目前尚无有效的控制策略。寻找用于开发抗 WSSV 药物的抗病毒先导化合物是紧迫且必要的；在这项研究中，来自 12 种单体化合物的七叶苷表现出优异的抗 WSSV 活性。结果表明，七叶苷将感染 WSSV 的虾的存活率提高了 59%，并在 100 μM 时将体内病毒拷贝数减少了 90%以上。在治疗前和治疗后实验中，七叶苷可以预防和治疗WSSV感染。与对照组相比，七叶苷与WSSV颗粒预孵育6 h后病毒拷贝数减少30%，在一定程度上抑制了WSSV的水平传播。考虑到七叶苷的抗病毒活性在养殖水中2天稳定，我们评估了连续用药变化的给药模式。显然，WSSV感染的虾在不更换七叶苷的情况下108 h的存活率为0%，而在连续换药的情况下，120 h的存活率超过40%。此外，七叶苷显着增加了抗菌肽的表达，从而提高了虾抵抗WSSV的能力。总的来说，我们的研究结果表明七叶苷有可能被开发成一种抗 WSSV 药物。而在 120 小时，连续换药的存活率超过 40%。此外，七叶苷显着增加了抗菌肽的表达，从而提高了虾抵抗WSSV的能力。总的来说，我们的研究结果表明七叶苷有可能被开发成一种抗 WSSV 药物。而在 120 小时，连续换药的存活率超过 40%。此外，七叶苷显着增加了抗菌肽的表达，从而提高了虾抵抗WSSV的能力。总的来说，我们的研究结果表明七叶苷有可能被开发成一种抗 WSSV 药物。

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Antiviral activity of esculin against white spot syndrome virus: A new starting point for prevention and control of white spot disease outbreaks in shrimp seedling culture

White spot syndrome virus (WSSV) is a pathogenic and threatening virus in shrimp culture for which there is no effective control strategy. Finding antiviral lead compounds for the development of anti-WSSV drugs is urgent and necessary; in this study, esculin from 12 monomeric compounds exhibited an excellent anti-WSSV activity. The results showed that esculin increased the survival rate of WSSV-infected shrimps by 59% and reduced the virus copy number in vivo over 90% at 100 Î¼M. In the pre-treatment and post-treatment experiments, esculin could prevent and treat WSSV infection. Compared with the control group, the virus copy number decreased by 30% after 6 h of esculin pre-incubation with WSSV particles and inhibited horizontal transmission of WSSV to a certain extent. Considering that the antiviral activity of esculin was stable in the aquacultural water for 2 days, we evaluated the dosing pattern of continuous medication changes. Obviously, the survival rate of WSSV-infected shrimps was 0% at 108 h when no esculin exchange was made, while at 120 h the survival rate was over 40% at continuous medicine changes. In addition, esculin significantly increased the expression of antimicrobial peptides and thus improved the ability of shrimp to resist WSSV. Overall, our findings suggest that esculin has the potential to be developed into an anti-WSSV medicine.

更新日期：2021-09-18

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