Journal of Nanostructure in Chemistry ( IF 8.6 ) Pub Date : 2021-09-13 , DOI: 10.1007/s40097-021-00439-0 Kumar Vishven Naveen 1 , Kandasamy Saravanakumar 1 , Arokia Vijaya Anand Mariadoss 1 , Myeong-Hyeon Wang 1 , Hye-Young Kim 2
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The present study designed to fabricate the silver nanoparticles from the aqueous leaf extracts of Potentilla chinensis Ser (Pc-AgNPs) and their physicochemical characterization were affirmed by spectroscopic and analytical techniques. The absorption maxima at 430 nm verified the synthesis of Pc-AgNPs. Further, the XRD spectroscopy authenticated the crystal, face-centered cubic (fcc) structure of Pc-AgNPs with the 2Ө value of 38.13°, 44.34°, 64.31° and 77.36°. FT-IR spectrum revealed the contribution of functional biomolecules in reduction of Ag+ to Ag0. Further the zeta potential, particle size and polydispersity index (PDI) of Pc-AgNPs were found as -36.6 ± 0.52 mV, 136 ± 24.82 nm and 0.202, respectively. Besides, the anisotropic structure of Pc-AgNPs with an average size of 24.3 ± 4.6 nm was confirmed by TEM analysis. In vitro antibacterial action of Pc-AgNPs was explored by agar well diffusion, then microtiter method were performed to detect the MIC and MBC of Pc-AgNPs. Further, the cellular changes in bacterial cells were visualized by confocal laser scanning and scanning electron microscopy. Pc-AgNPs considerably inhibited the growth of Salmonella enterica, Staphylococcus aureus, Bacillus cereus and Escherichia coli. The minimum inhibitory concentration (MIC) of Pc-AgNPs was found for S. aureus (5.0 ± 1.15 µg/mL), B. cereus (6.25 ± 0.58 µg/mL), S. enterica (3.75 ± 0.73 µg/mL) and E. coli (3.12 ± 1.25 µg/mL). Besides the biocompatible nature of Pc-AgNPs was explored in HEK-293 cell line, which revealed that up to 500 µg/mL did not cause any significant toxicity. Taken together, our results conclude that Pc-AgNPs have the potential antibacterial activity with promising biocompatibility in HEK-293 cells.
Graphic abstract
中文翻译：
使用 Potentilla chinensis Ser 叶的生物相容性银纳米粒子的植物制造：其抗菌活性的表征和评价
本研究旨在从Potentilla chinensis Ser (Pc-AgNPs)的水叶提取物中制备银纳米粒子，并通过光谱和分析技术对其物理化学表征进行了确认。430 nm 处的吸收最大值验证了 Pc-AgNPs 的合成。此外，XRD 光谱验证了 Pc-AgNPs 的晶体、面心立方 (fcc) 结构，其 2Ө 值为 38.13°、44.34°、64.31° 和 77.36°。FT-IR 光谱揭示了功能性生物分子在将 Ag +还原为 Ag 0 方面的贡献. 此外，Pc-AgNPs 的 zeta 电位、粒径和多分散指数 (PDI) 分别为 -36.6 ± 0.52 mV、136 ± 24.82 nm 和 0.202。此外，TEM 分析证实了平均尺寸为 24.3 ± 4.6 nm 的 Pc-AgNPs 的各向异性结构。通过琼脂孔扩散法考察了Pc-AgNPs的体外抗菌作用，然后用微量滴定法检测了Pc-AgNPs的MIC和MBC。此外，通过共聚焦激光扫描和扫描电子显微镜观察细菌细胞中的细胞变化。Pc-AgNPs显着抑制了沙门氏菌、金黄色葡萄球菌、蜡状芽孢杆菌和大肠杆菌的生长. 发现 Pc-AgNPs 的最小抑制浓度 (MIC) 对金黄色葡萄球菌(5.0 ± 1.15 µg/mL)、蜡状芽孢杆菌(6.25 ± 0.58 µg/mL)、S. enterica (3.75 ± 0.73 µg/mL) 和大肠杆菌(3.12 ± 1.25 µg/mL)。此外，在 HEK-293 细胞系中探索了 Pc-AgNPs 的生物相容性，结果表明高达 500 µg/mL 不会引起任何显着毒性。总之，我们的结果得出结论，Pc-AgNPs 具有潜在的抗菌活性，在 HEK-293 细胞中具有良好的生物相容性。
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