The self-stratification is an ecologic approach providing the solvent emission reduction and formation of a laminated multi-functional structure by one-coat application. Although light-curing systems as a point of interest have been developed extensively, the self-stratifying phenomena and its governing parameters have not been investigated in such a fast-curable coating. This study aimed to design solvent-free and light-curable coatings with various self-stratifying behavior using methacrylated gelatin (GelMA) and acrylated epoxidized soybean oil (AESO). The influences of several essential factors including flash-off time, irradiation time, light intensity, photoinitiator concentration (PI%), coating thickness, and raw materials weight ratio on the stratification level were evaluated by SEM-EDX, AFM, ATR-FTIR spectroscopy, water contact angle (WCA), and conventional coating analyses. It was learned that about 10 min of flash-off time was required to start the phase separation and create gradient integrated layered structures. Also, the results demonstrated that the self-stratification was not impressed by prolonging the irradiation time after the formation of the lattice structure. Furthermore, increasing the coating thickness to 300 μm or decreasing it to 30 μm led to uncured or slightly gradient stratified coatings, respectively. Besides, the lower GelMA content resulted in the lower mixture viscosity and sharper gradient self-stratified structures. Moreover, the increasing of crosslinking rate by PI% or light intensity increment changed the bilayer stratified structure into the gradient ones; therefore, self-stratifying in this fast-curing system was both thermodynamically and kinetically controlled. Subsequently, the know-how for preparing different self-stratified coatings with various features, namely swelling degree (11–29%), gel fraction (74–92%), glass transition temperature (43–72 °C), WCA (67.1–81.6°), MEK double rub resistance (25–55), pendulum hardness (45–83), cross-cut adhesion (0B4B), gloss (53–87 at 60°), and surface roughness (72.8–235.7 nm) was achieved. Additionally, the relation of the surface GelMA content and the surface roughness, gloss, hardness, and WCA were derived. Accordingly, this attempt gave a helpful insight into the self-stratification phenomena to establish the optimum formulation and processing conditions as a promising prospect to prepare green coatings for advanced bio and food applications.

自分层是一种生态方法，可通过单涂层应用减少溶剂排放并形成层压多功能结构。尽管光固化系统作为一个关注点已经得到广泛的开发，但尚未对这种快速固化涂层中的自分层现象及其控制参数进行研究。本研究旨在使用甲基丙烯酸化明胶 (GelMA) 和丙烯酸化环氧化大豆油 (AESO) 设计具有各种自分层行为的无溶剂和光固化涂料。通过SEM-EDX、AFM、ATR-FTIR光谱评估闪蒸时间、照射时间、光强度、光引发剂浓度（PI%）、涂层厚度和原料重量比等几个主要因素对分层水平的影响, 水接触角 (WCA) 和传统涂层分析。据了解，需要大约 10 分钟的闪蒸时间来开始相分离并产生梯度集成层状结构。此外，结果表明，在形成晶格结构后延长照射时间不会影响自分层。此外，将涂层厚度增加到 300 μm 或将其减小到 30 μm，分别导致未固化或略微梯度分层的涂层。此外，较低的 GelMA 含量导致较低的混合物粘度和更陡峭的梯度自分层结构。此外，交联率增加PI%或光强增加使双层分层结构变为梯度结构；所以，这种快速固化系统中的自分层受热力学和动力学控制。随后，制备具有各种特性的不同自分层涂层的专有技术，即溶胀度 (11-29%)、凝胶分数 (74-92%)、玻璃化转变温度 (43-72 °C)、WCA (67.1 –81.6°)、MEK 双重耐磨性 (25–55)、摆锤硬度 (45–83)、划格附着力 (0B)4B)、光泽度（53-87 在 60°）和表面粗糙度（72.8-235.7 nm）。此外，还推导出了表面 GelMA 含量与表面粗糙度、光泽度、硬度和 WCA 的关系。因此，这一尝试对自分层现象提供了有益的见解，以建立最佳配方和加工条件，作为制备用于先进生物和食品应用的绿色涂料的有希望的前景。