Rapid Detection of SARS-CoV-2 Antigens and Antibodies Using OFET Biosensors Based on a Soft and Stretchable Semiconducting Polymer,ACS Biomaterials Science & Engineering

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Rapid Detection of SARS-CoV-2 Antigens and Antibodies Using OFET Biosensors Based on a Soft and Stretchable Semiconducting Polymer
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2021-09-14 , DOI: 10.1021/acsbiomaterials.1c00727
Kristina Ditte _{1,

2} , Trang Anh Nguyen Le _{3,

4} , Oliver Ditzer _{1,

2} , Diana Isabel Sandoval Bojorquez ₃ , Soosang Chae ₁ , Michael Bachmann _{3,

4} , Larysa Baraban ₃ , Franziska Lissel _{1,

2,

5}

Affiliation

In the midst of the COVID-19 pandemic, adaptive solutions are needed to allow us to make fast decisions and take effective sanitation measures, e.g., the fast screening of large groups (employees, passengers, pupils, etc.). Although being reliable, most of the existing SARS-CoV-2 detection methods cannot be integrated into garments to be used on demand. Here, we report an organic field-effect transistor (OFET)-based biosensing device detecting of both SARS-CoV-2 antigens and anti-SARS-CoV-2 antibodies in less than 20 min. The biosensor was produced by functionalizing an intrinsically stretchable and semiconducting triblock copolymer (TBC) film either with the anti-S1 protein antibodies (S1 Abs) or receptor-binding domain (RBD) of the S1 protein, targeting CoV-2-specific RBDs and anti-S1 Abs, respectively. The obtained sensing platform is easy to realize due to the straightforward fabrication of the TBC film and the utilization of the reliable physical adsorption technique for the molecular immobilization. The device demonstrates a high sensitivity of about 19%/dec and a limit of detection (LOD) of 0.36 fg/mL for anti-SARS-Cov-2 antibodies and, at the same time, a sensitivity of 32%/dec and a LOD of 76.61 pg/mL for the virus antigen detection. The TBC used as active layer is soft, has a low modulus of 24 MPa, and can be stretched up to 90% with no crack formation of the film. The TBC is compatible with roll-to-roll printing, potentially enabling the fabrication of low-cost wearable or on-skin diagnostic platforms aiming at point-of-care concepts.

中文翻译：

使用基于柔软可拉伸半导体聚合物的 OFET 生物传感器快速检测 SARS-CoV-2 抗原和抗体

在 COVID-19 大流行期间，需要适应性解决方案，使我们能够快速做出决定并采取有效的卫生措施，例如，对大量人群（员工、乘客、学生等）进行快速筛查。尽管可靠，但大多数现有的 SARS-CoV-2 检测方法无法集成到按需使用的服装中。在这里，我们报告了一种基于有机场效应晶体管 (OFET) 的生物传感设备，可在不到 20 分钟的时间内检测 SARS-CoV-2 抗原和抗 SARS-CoV-2 抗体。该生物传感器是通过使用抗 S1 蛋白抗体 (S1 Abs) 或 S1 蛋白的受体结合域 (RBD) 功能化本质上可拉伸的半导体三嵌段共聚物 (TBC) 薄膜而制成的，靶向 CoV-2 特异性 RBD 和分别为抗 S1 抗体。由于 TBC 薄膜的直接制造和利用可靠的物理吸附技术进行分子固定，所获得的传感平台易于实现。该装置对抗 SARS-Cov-2 抗体表现出约 19%/dec 的高灵敏度和 0.36 fg/mL 的检测限 (LOD)，同时具有 32%/dec 的灵敏度和病毒抗原检测的 LOD 为 76.61 pg/mL。用作活性层的 TBC 柔软，具有 24 MPa 的低模量，并且可以拉伸高达 90% 而不会形成薄膜裂纹。TBC 与卷对卷打印兼容，有可能实现针对即时护理概念的低成本可穿戴或皮肤诊断平台的制造。

更新日期：2021-09-14

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