Effects of Folic Acid and Caffeic Acid on Indirect Photo-oxidation of Proteins and Their Costabilization under Irradiation,Journal of Agricultural and Food Chemistry

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Effects of Folic Acid and Caffeic Acid on Indirect Photo-oxidation of Proteins and Their Costabilization under Irradiation
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-09-14 , DOI: 10.1021/acs.jafc.1c02209
Wusigale _{1,

2,

3,

4} , Xiaojun Fu _{1,

2} , Xin Yin _{1,

2} , Chuye Ji _{1,

2} , Hao Cheng _{1,

2} , Li Liang _{1,

2}

Affiliation

Proteins, vitamins, and phenols are often present together in foods, but they are sensitive to environmental factors. Folic acid (FA), a synthetic form of folate, decomposes under light, leading to protein oxidation. Caffeic acid (CA), a phenolic acid, exhibits remarkable activity for scavenging reactive molecules. The exploitation of their interactions offers opportunities for designing the stabilizing system. In this study, FA-photodecomposition-induced protein (β-lactoglobulin, α-lactalbumin, bovine serum albumin, and β-casein) damage and its inhibition by CA were investigated in terms of protein composition and structural change. The results indicated that FA photoproducts oxidized the proteins via the electron transfer pathway, leading to degradation, aggregation, and unfolding. At the same time, photostability of FA, CA, and proteins in the tertiary mixture was better than that of any individual components. The antioxidant activity of the proteins contributed to their protection for FA. CA and its products inhibited FA photodecomposition and the photodecomposition-induced protein oxidation by trapping excited states.

中文翻译：

叶酸和咖啡酸对蛋白质间接光氧化及其辐照共稳定的影响

蛋白质、维生素和酚类通常同时存在于食物中，但它们对环境因素很敏感。叶酸 (FA) 是一种合成形式的叶酸，在光照下会分解，导致蛋白质氧化。咖啡酸 (CA) 是一种酚酸，对清除反应性分子具有显着的活性。利用它们的相互作用为设计稳定系统提供了机会。本研究从蛋白质组成和结构变化方面研究了 FA 光分解诱导的蛋白质（β-乳球蛋白、α-乳清蛋白、牛血清白蛋白和 β-酪蛋白）损伤及其抑制作用。结果表明 FA 光产物通过以下方式氧化蛋白质电子转移途径，导致降解、聚集和展开。同时，三级混合物中 FA、CA 和蛋白质的光稳定性优于任何单个组分。蛋白质的抗氧化活性有助于它们对 FA 的保护。CA 及其产物通过捕获激发态来抑制 FA 光分解和光分解诱导的蛋白质氧化。

更新日期：2021-10-27

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