棕榈树Xylariale内生真菌与植物病原菌尖孢镰刀菌拮抗化合物的鉴定,Journal of Agricultural and Food Chemistry

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棕榈树Xylariale内生真菌与植物病原菌尖孢镰刀菌拮抗化合物的鉴定
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-09-13 , DOI: 10.1021/acs.jafc.1c03141
Morgane Barthélemy ₁ , Nicolas Elie ₁ , Grégory Genta-Jouve ₂ , Didier Stien ₃ , David Touboul ₁ , Véronique Eparvier ₁

Affiliation

为发现天然具有对抗植物病原体尖孢镰刀菌的化学武器的微生物，研究了植物叶片内生菌的生物和化学多样性。从法属圭亚那原始森林中收集的棕榈树Astrocaryum sciophyllum中分离出内生菌。几种木兰科抑制尖孢镰刀菌的生长并进行了进一步探索。从与植物病原体对抗的木香科 BSNB-0294 菌株中分离出抗真菌专用代谢物，并鉴定出未描述的化合物，即两种名为木香素的缩肽、两种含有 3-咪唑啉酮部分的代谢物和四种新化合物，包括硝基-苯丙酰胺和三种苯丙氨酸类似物命名为木兰素 A-D。同时，在共培养过程中对植物病原体的化学研究导致了一种未知化合物的鉴定，我们将其命名为 focicin。比赛期间焦素的产生加剧。基质辅助激光解吸/电离耦合到飞行时间质谱 (MALDI-TOF MS) 成像 BSNB-0294（内生菌株）和尖孢镰刀菌之间的竞争f.sp. ciceris（植物病原体）突出了两种微生物之间的时间依赖性化学相互作用。

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Identification of Antagonistic Compounds between the Palm Tree Xylariale Endophytic Fungi and the Phytopathogen Fusarium oxysporum

To discover microorganisms that naturally possess chemical weapons against the phytopathogen Fusarium oxysporum, the biological and chemical diversity of plant leaf endophytes was investigated. Endophytes were isolated from the palm tree Astrocaryum sciophyllum collected in pristine forests of French Guiana. Several Xylariaceae inhibited the growth of F. oxysporum and were further explored. Antifungal specialized metabolites were isolated from the Xylariaceae BSNB-0294 strain in confrontation with the phytopathogen and led to the identification of undescribed compounds, i.e., two depsipeptides named xylariaceins, two metabolites containing a 3-imidazolinone moiety, and four new compounds including a nitro-phenylpropanamide and three phenylalanine analogues named xylariains A–D. In parallel, the chemical investigation of the phytopathogen during the coculture led to the identification of an unknown compound, which we named focicin. The production of focicin was exacerbated during the competition. Matrix-assisted laser desorption/ionization coupled to time-of-flight mass spectometry (MALDI-TOF MS) imaging of the competition between BSNB-0294 (endophytic strain) and F. oxysporum f.sp. ciceris (phytopathogen) highlighted time-dependent chemical interactions between the two microorganisms.

更新日期：2021-09-22

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