<br>在 Fe2+ 和 KI 的帮助下，通过 ABTS 的氧化着色法测定过氧乙酸和共存的过氧化氢,Chemosphere

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在 Fe2+ 和 KI 的帮助下，通过 ABTS 的氧化着色法测定过氧乙酸和共存的过氧化氢
Chemosphere ( IF 8.1 ) Pub Date : 2021-09-11 , DOI: 10.1016/j.chemosphere.2021.132242
Jinbin Lin ₁ , Junyang Xiao ₂ , Hengyu Cai ₁ , Yixin Huang ₁ , Jiawen Li ₁ , Haoyu Yang ₁ , Tao Li ₃ , Jing Zou ₁

Affiliation

本研究提出了一种同时测定过氧乙酸（PAA）和共存过氧化氢（H2O2）的多波长分光光度法。该方法基于在 Fe2+/KI 的帮助下氧化 2,2′-联氮基-双（3-乙基苯并噻唑啉-6-磺酸盐）（ABTS），生成稳定的绿色自由基（ABTS●+），可在四个特征峰（即 415 nm、650 nm、732 nm 和 820 nm）处测定。ABTS●+ 在四个峰处的吸光度呈良好线性（R2 > 0.999），在优化条件下，总过氧化物（PAA + H2O2）和 PAA 的浓度在 0–40 μM 范围内。测定 415 nm、650 nm、732 nm 和 820 nm 处总过氧化物的灵敏度分别为 4.248 × 104 M-1 cm-1、1.682 × 104 M-1 cm-1、2.132 × 104 M-1 cm-1 和 1.928 × 104 M-1 cm-1。对于测定 PAA，相应的灵敏度分别为 4.622 × 104 M-1 cm-1、1.895 × 104 M-1 cm-1、2.394 × 104 M-1 cm-1 和 2.153 × 104 M-1 cm-1。共存的 H 2 O 2 的浓度是通过从总过氧化物浓度中减去 PAA 浓度来获得的。ABTS 方法足够准确，可以测定天然水样中的 PAA 浓度。此外，ABTS 方法成功地用于确定 PAA 和共存 H2O2 的变化，并区分它们在热活化 PAA 过程中对萘普生降解的作用。总体而言，ABTS 方法可以用作方便、快速、灵敏地测定水样中 PAA 和共存 H2O2 的替代方法。

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Multi-wavelength spectrophotometric determination of peracetic acid and the coexistent hydrogen peroxide via oxidative coloration of ABTS with the assistance of Fe2+ and KI

In this study, a multi-wavelength spectrophotometric method for simultaneous determination of peracetic acid (PAA) and coexistent hydrogen peroxide (H2O2) was presented. This method was based on the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) with the assistance of Fe2+/KI to produce a stable green radical (ABTS●+), which could be determined at four characteristic peaks (i.e., 415 nm, 650 nm, 732 nm, and 820 nm). The absorbances of ABTS●+ at four peaks were well linear (R2 > 0.999) with concentrations of both total peroxides (PAA + H2O2) and PAA in the range of 0–40 μM under optimized conditions. The sensitivities for determining total peroxides at 415 nm, 650 nm, 732 nm and 820 nm were determined to be 4.248 × 104 M−1 cm−1, 1.682 × 104 M−1 cm−1, 2.132 × 104 M−1 cm−1, and 1.928 × 104 M−1 cm−1, respectively. For determining PAA, the corresponding sensitivities were 4.622 × 104 M−1 cm−1, 1.895 × 104 M−1 cm−1, 2.394 × 104 M−1 cm−1 and 2.153 × 104 M−1 cm−1, respectively. The concentration of coexistent H2O2 was gained by deducting PAA concentration from total peroxides concentration. The ABTS method was accurate enough to determine PAA concentration in natural water samples. Moreover, the ABTS method was successfully used to determine the changes of PAA and coexistent H2O2 and to distinguish their role on naproxen degradation in heat-activated PAA process. Overall, the ABTS method could be used as an alternative method for the convenient, rapid and sensitive determination of PAA and the coexistent H2O2 in water samples.

更新日期：2021-09-11

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