Ethanolic extract of Pyrus pashia buch ham ex. D. Don (Kainth): A bioaccessible source of polyphenols with anti-inflammatory activity in vitro and in vivo,Journal of Ethnopharmacology

当前位置： X-MOL 学术 › J. Ethnopharmacol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Ethanolic extract of Pyrus pashia buch ham ex. D. Don (Kainth): A bioaccessible source of polyphenols with anti-inflammatory activity in vitro and in vivo
Journal of Ethnopharmacology ( IF 4.8 ) Pub Date : 2021-09-11 , DOI: 10.1016/j.jep.2021.114628
Prakash Om ₁ , M S Gopinath ₂ , P Madan Kumar ₃ , S P Muthu Kumar ₃ , V B Kudachikar ₁

Affiliation

Ethnopharmacological relevance

Pyrus pashia Buch ham ex. D. Don (Kainth) fruit from the Himalayan region is traditionally consumed by native people in the form of decoctions for various clinical conditions including inflammatory diseases. However, scientific studies on the biofunctional properties of Kainth fruits are still scarce.

Aim of the study: The study is aimed to investigate the anti-inflammatory effects of Kainth fruit extracts using in vitro and in vivo inflammation models.

Material and methods

Free, esterified and bound fractions from the Kainth ethanolic extracts were prepared for determining the anti-inflammatory effect. The levels of 5-LOX and COX-2 were determined in vitro. The protein levels of cytokines (IL-6, TNF-α & IL-10) were quantitated by ELISA method in lipopolysaccharide-stimulated RAW macrophages. Also, the anti-inflammatory potential of the Kainth fruit extracts was determined using the carrageenan-induced mice paw edema model. The bioaccessibility of Kainth fruit extracts was measured using a simulated in vitro digestion system (salivary, gastric and intestinal).

Results

The Kainth fruit extracts were partially purified to yield free, esterified and bound phenolics. Free and bound phenolics of Kainth fruits inhibited 5-Lipoxygenase, Cyclooxygenase-2 activities and pro-inflammatory cytokines (Interleukin-6 and tumour necrosis factor-α) expression in vitro. Also, oral administration of these extracts to the carrageenan-injected mice showed an anti-inflammatory effect by decreasing the pro-inflammatory cytokines and reducing the cellular infiltration in paw tissues. Also, both the extracts showed better bioavailability and bioaccessibility in in vitro and in vivo studies.

Conclusions

The results indicated that free and bound phenolics from Kainth fruits that are rich in catechin, epicatechin, arbutin and chlorogenic acid exhibited anti-inflammatory effects and could potentially be used to treat inflammatory diseases.

中文翻译：

Pyrus pashia buch ham ex 的乙醇提取物。D. Don (Kainth)：具有体外和体内抗炎活性的生物可及的多酚来源

民族药理学相关性

Pyrus pashia Buch 火腿 ex。D. 来自喜马拉雅地区的唐 (Kainth) 水果传统上被当地人以煎剂的形式食用，用于治疗各种临床疾病，包括炎症性疾病。然而，关于 Kainth 水果的生物功能特性的科学研究仍然很少。

研究目的：该研究旨在使用体外和体内炎症模型研究 Kainth 水果提取物的抗炎作用。

材料与方法

制备来自 Kainth 乙醇提取物的游离、酯化和结合部分，用于确定抗炎作用。体外测定了 5-LOX 和 COX-2 的水平。通过 ELISA 方法在脂多糖刺激的 RAW 巨噬细胞中定量细胞因子 (IL-6、TNF-α 和 IL-10) 的蛋白质水平。此外，使用角叉菜胶诱导的小鼠爪水肿模型确定了 Kainth 果实提取物的抗炎潜力。使用模拟的体外消化系统（唾液、胃和肠）测量 Kainth 水果提取物的生物可及性。

结果

Kainth 果实提取物被部分纯化以产生游离、酯化和结合的酚类物质。Kainth 果实的游离和结合酚类物质在体外抑制 5-脂氧合酶、环氧合酶-2 活性和促炎细胞因子（白细胞介素 6 和肿瘤坏死因子-α）的表达。此外，将这些提取物口服给予注射角叉菜胶的小鼠通过减少促炎细胞因子和减少爪组织中的细胞浸润显示出抗炎作用。此外，两种提取物在体外和体内研究中均显示出更好的生物利用度和生物可及性。