In this study, various combined pretreatment strategies using liquid hot water (LHW), 60 % ethanol, 1% H₂SO₄ and 0.5 % NaOH together with different surfactants (PEG 6000, Triton X-100 and Tween 80) were evaluated to improve glucose production from sugarcane bagasse (SCB) during enzymatic hydrolysis. After pretreatment with PEG 6000-assisted 1% H₂SO₄, the highest glucose yield of 75.04 % was achieved. Pretreatments of SCB with various combinations of H₂SO₄ (1–3%, w/v) and PEG 6000 (0–1.5 %, w/v) were further investigated for enhancing enzymatic hydrolysis and ethanol production. When 1.5 % PEG 6000 was introduced during pretreatment with 2 % H₂SO₄, the highest glucose yield of 86.14 % and ethanol concentration of 20.17 g/L were liberated, which were much higher than those obtained from pretreatment with 2% H₂SO₄ alone. This increased digestibility and fermentability can be attributed to decreases in crystalline sizes and PEG 6000 adherence to SCB surfaces, which was confirmed by cellulase adsorption isotherms, FT-IR, XRD, and SEM analysis. In addition, prehydrolysate and the stillage remaining after ethanol production were sequentially digested to seek potential biomethane production and resulted in final methane yields of 264.05 mL and 387.77 mL per g of volatile solids, respectively. The feasibility of the process provids an environmentally friendly technology for an integrated biorefinery.

在本研究中，对使用液态热水 (LHW)、60% 乙醇、1% H ₂ SO ₄和 0.5% NaOH 以及不同表面活性剂（PEG 6000、Triton X-100 和 Tween 80）的各种组合预处理策略进行了评估，以改善甘蔗渣（SCB）在酶促水解过程中的葡萄糖生产。在用 PEG 6000 辅助的 1% H ₂ SO ₄预处理后，达到了 75.04% 的最高葡萄糖产率。进一步研究了用 H ₂ SO ₄ (1–3%, w/v) 和 PEG 6000 (0–1.5%, w/v) 的各种组合预处理 SCB 以增强酶水解和乙醇生产。在使用 2 % H ₂ SO预处理期间引入 1.5 % PEG 6000 时_{如图4所示}，最高葡萄糖产率86.14%，乙醇浓度20.17g/L，远高于单独用2%H ₂ SO ₄预处理所得的结果。这种增加的消化率和发酵性可归因于结晶尺寸的减少和 PEG 6000 对 SCB 表面的粘附，纤维素酶吸附等温线、FT-IR、XRD 和 SEM 分析证实了这一点。此外，乙醇生产后剩余的预水解物和酒糟液依次消化以寻找潜在的生物甲烷生产，最终甲烷产量分别为每克挥发性固体 264.05 毫升和 387.77 毫升。该工艺的可行性为综合生物精炼厂提供了一种环保技术。