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Involvement of Chemosensory Protein BodoCSP1 in Perception of Host Plant Volatiles in Bradysia odoriphaga
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-09-10 , DOI: 10.1021/acs.jafc.1c02807 Chunni Zhang 1 , Bowen Tang 1 , Taoling Zhou 1 , Xiaoting Yu 1 , Manfei Hu 1 , Wu Dai 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-09-10 , DOI: 10.1021/acs.jafc.1c02807 Chunni Zhang 1 , Bowen Tang 1 , Taoling Zhou 1 , Xiaoting Yu 1 , Manfei Hu 1 , Wu Dai 1
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Chemosensory proteins (CSPs) can bind and transport odorant molecules and play important roles in insect chemoreception. In this study, we focused on the roles of a chemosensory protein (BodoCSP1) in perception of host plant volatiles in Bradysia odoriphaga. The expression of BodoCSP1 was significantly higher in adults than in larvae and pupae, without a significant difference between male and female adults. Recombinant protein BodoCSP1 exhibited relatively high binding affinities to 9 out of 10 tested ligands (Ki < 10 μM). Behavioral assays revealed that adults of B. odoriphaga showed a significant preference for five compounds. The predicted three-dimensional (3D) structure of BodoCSP1 has the typical six α-helices that form the hydrophobic ligand-binding pocket. Molecular docking and site-directed mutagenesis combined with ligand-binding assays indicated that Val48 and Thr66 may be the key binding site in BodoCSP1 for host plant volatiles. RNAi results indicated that dsBodoCSP1-treated adults showed significant reductions in response to diallyl disulfide, dipropyl disulfide, and allyl methyl disulfide. These results indicated that BodoCSP1 plays essential functions in the perception of host plant volatiles in B. odoriphaga.
中文翻译：
化学感应蛋白 BodoCSP1 参与对臭蚧中寄主植物挥发物的感知
化学感应蛋白 (CSP) 可以结合和运输气味分子，并在昆虫化学感受中发挥重要作用。在这项研究中，我们专注于化学感应蛋白 (BodoCSP1) 在Bradysia odoriphaga中宿主植物挥发物的感知中的作用。BodoCSP1在成虫中的表达显着高于幼虫和蛹，雄虫和雌虫之间无显着差异。重组蛋白 BodoCSP1 对 10 个测试配体中的 9 个表现出相对较高的结合亲和力（K i < 10 μM）。行为分析表明，B. odoriphaga 的成虫显示出对五种化合物的显着偏好。BodoCSP1 的预测三维 (3D) 结构具有典型的六个 α-螺旋，形成疏水性配体结合口袋。分子对接和定点诱变结合配体结合试验表明，Val48 和 Thr66 可能是 BodoCSP1 中宿主植物挥发物的关键结合位点。RNAi 结果表明，dsBodoCSP1处理的成年人对二烯丙基二硫化物、二丙基二硫化物和烯丙基甲基二硫化物的反应显着降低。这些结果表明，BodoCSP1 在B. odoriphaga 中对寄主植物挥发物的感知中起重要作用。
更新日期：2021-09-22
中文翻译：
化学感应蛋白 BodoCSP1 参与对臭蚧中寄主植物挥发物的感知
化学感应蛋白 (CSP) 可以结合和运输气味分子，并在昆虫化学感受中发挥重要作用。在这项研究中，我们专注于化学感应蛋白 (BodoCSP1) 在Bradysia odoriphaga中宿主植物挥发物的感知中的作用。BodoCSP1在成虫中的表达显着高于幼虫和蛹，雄虫和雌虫之间无显着差异。重组蛋白 BodoCSP1 对 10 个测试配体中的 9 个表现出相对较高的结合亲和力（K i < 10 μM）。行为分析表明，B. odoriphaga 的成虫显示出对五种化合物的显着偏好。BodoCSP1 的预测三维 (3D) 结构具有典型的六个 α-螺旋，形成疏水性配体结合口袋。分子对接和定点诱变结合配体结合试验表明，Val48 和 Thr66 可能是 BodoCSP1 中宿主植物挥发物的关键结合位点。RNAi 结果表明，dsBodoCSP1处理的成年人对二烯丙基二硫化物、二丙基二硫化物和烯丙基甲基二硫化物的反应显着降低。这些结果表明，BodoCSP1 在B. odoriphaga 中对寄主植物挥发物的感知中起重要作用。
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