长链非编码 RNA CASC2 通过 miR-18a/SIRT2 保护 ROS 诱导的心肌梗死氧化应激,Biotechnology and Applied Biochemistry

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长链非编码 RNA CASC2 通过 miR-18a/SIRT2 保护 ROS 诱导的心肌梗死氧化应激
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2021-09-10 , DOI: 10.1002/bab.2252
Guoqiu Ying _{1,

2} , Zijun Tang ₂ , Jing Zhang ₂ , Junyi Zeng _{1,

2,

3} , Zeqi Zheng _{1,

2,

3} , Wan Zhang _{2,

3} , Lu Ding _{2,

3} , Tong Wen _{1,

2} , Dasong Yi _{1,

2}

Affiliation

我们旨在研究长链非编码 RNA (lncRNA) 在急性心肌梗死 (AMI) 模型中的功能及其可能机制。AMI患者和正常志愿者均选自我院。将 Sprague-Dawley 大鼠诱导成 AMI 体内模型。H9c2细胞用H ₂ O _2处理生成伤害模型。检测到 lncRNA CASC2 的血清基因表达显着降低。在AMI大鼠模型中，AMI小鼠心脏组织lncRNA CASC2基因表达降低。在体外模型中，lncRNA CASC2 的下调增加了活性氧 (ROS) 诱导的氧化应激；lncRNA CASC2诱导NADPH氧化酶（NOX-2）表达并抑制miR-18a表达；MiR-18a 促进 ROS 诱导的氧化应激；miR-18a 的下调降低了 ROS 诱导的氧化应激。miR-18a 的抑制逆转了 CASC2 下调对 AMI 体外模型中 ROS 诱导的氧化应激的影响。miR-18a 的激活逆转了 CASC2 对 AMI 体外模型中 ROS 诱导的氧化应激的影响。这些数据首次提示lncRNA CASC2对AMI具有更好的保护作用，

"点击查看英文标题和摘要"

Long noncoding RNA CASC2 protect ROS-induced oxidative stress in myocardial infarction by miR-18a/SIRT2

We aimed to investigate the function and its possible mechanisms of long noncoding RNA (lncRNA) in acute myocardial infarction (AMI) model. Patients with AMI and normal volunteers were selected from our hospital. Sprague–Dawley rats were induced into in vivo model of AMI. H9c2 cells were treated with H₂O₂ to generate injury model. A significantly lower serum gene expression of lncRNA CASC2 was detected. In rat models of AMI, lncRNA CASC2 gene expressions in heart tissue of mice with AMI were decreased. In in vitro model, downregulation of lncRNA CASC2 increased reactive oxygen species (ROS)-induced oxidative stress; lncRNA CASC2 induced NADPH oxidase (NOX-2) expression and suppressed miR-18a expression; MiR-18a promoted ROS-induced oxidative stress; downregulation of miR-18a decreased ROS-induced oxidative stress. The inhibition of miR-18a reversed the effects of CASC2 downregulation on ROS-induced oxidative stress in in vitro model of AMI. The activation of miR-18a reversed the effects of CASC2 on ROS-induced oxidative stress in in vitro model of AMI. These data for the first time suggest that lncRNA CASC2 have better protective effects on AMI, which could reduce oxidative stress through their carried miR-18a and subsequently downregulating the SIRT2/ROS pathway.

更新日期：2021-09-10

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