The emerging field of DNA nanotechnology harnesses DNA as a stable and generic material to assemble nanostructures with precisely defined sizes and geometries. The past 40 years have witnessed this field’s originating, branching, blossoming, bridging with, and benefiting many different areas. Interestingly, in nearly the same period, DNA has also been discovered to be able to catalyze various chemical reactions. The catalytic DNAs, especially those that can cleave DNA/RNA, are with a destructive property quite opposite to the fundamental concept of DNA nanotechnology. Nevertheless, in the past decade, they have been successfully blended into DNA nanoconstruction and expanded the construction dimension. Here, we present an overview of recent progress in the development of DNA nanotechnology promoted by the DNA/RNA-cleaving DNAs. Meanwhile, we provide an outlook of how the two seemingly distant types of DNA could further collide with and mutually benefit each other, leading to the unprecedented downstream applications in various areas.

DNA 纳米技术的新兴领域利用 DNA 作为一种稳定的通用材料来组装具有精确定义的尺寸和几何形状的纳米结构。40年来，这个领域见证了许多不同领域的起源、分支、开花、衔接和受益。有趣的是，几乎在同一时期，DNA 也被发现能够催化各种化学反应。催化性 DNA，尤其是那些可以切割 DNA/RNA 的 DNA，具有与 DNA 纳米技术的基本概念完全相反的破坏性。尽管如此，在过去十年中，它们已成功融入DNA纳米结构并扩展了结构维度。在这里，我们概述了由 DNA/RNA 切割 DNA 推动的 DNA 纳米技术发展的最新进展。同时，