外源性 HSP70 减轻硝酸甘油诱导的小鼠偏头痛样症状,Journal of Neurophysiology

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外源性 HSP70 减轻硝酸甘油诱导的小鼠偏头痛样症状
Journal of Neurophysiology ( IF 2.1 ) Pub Date : 2021-09-01 , DOI: 10.1152/jn.00314.2021
Fengfang Wu ₁ , Yifeng Huang ₂ , Guifang Wei ₁ , Zhiwei Huang ₁ , Congwen Shi ₁ , Weinian Lin ₁ , Jun Liao ₁

Affiliation

尽管热休克蛋白 70 (HSP70) 与前庭偏头痛之间的联系尚不清楚，但 HSP70 在其他情况下具有神经保护作用。本研究旨在研究外源性 HSP70 在硝酸甘油 (NTG) 诱导的偏头痛小鼠模型中治疗偏头痛样症状的潜力。通过ELISA评估前庭偏头痛患者和健康个体的HSP70水平。NTG 在小鼠中诱导偏头痛，并在用 NTG 和 NTG 以及外源 HSP70 处理的小鼠的三叉神经尾核 (TNC) 组织中检测 HSP70 的表达。通过行为分析评估外源性 HSP70 对偏头痛样症状的影响。最后，研究了 HSP70 对偏头痛小鼠氧化应激和 NF-κB 信号传导的影响。前庭性偏头痛患者血清HSP70明显低于健康人。NTG 给药显着抑制了小鼠 TNC 组织中 HSP70 的表达，而外源性 HSP70 逆转了这种表达。HSP70 减轻了 NTG 引起的机械超敏反应、光厌恶和焦虑样行为。最后，外源性 HSP70 抑制 NTG 诱导的氧化应激和 NF-κB 信号传导。我们的研究表明，外源性 HSP70 可能是缓解偏头痛症状的潜在疗法，我们有希望的发现值得进一步研究 HSP70 的临床应用。最后，外源性 HSP70 抑制 NTG 诱导的氧化应激和 NF-κB 信号传导。我们的研究表明，外源性 HSP70 可能是缓解偏头痛症状的潜在疗法，我们有希望的发现值得进一步研究 HSP70 的临床应用。最后，外源性 HSP70 抑制 NTG 诱导的氧化应激和 NF-κB 信号传导。我们的研究表明，外源性 HSP70 可能是缓解偏头痛症状的潜在疗法，我们有希望的发现值得进一步研究 HSP70 的临床应用。

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Exogenous HSP70 attenuates nitroglycerin-induced migraine-like symptoms in mice

Although the connection between heat shock protein 70 (HSP70) and vestibular migraine is not clear, HSP70 is neuroprotective in other scenarios. This study aimed to investigate the potential of exogenous HSP70 for treating migraine-like symptoms in a mouse model of nitroglycerin (NTG) induced migraine. HSP70 levels were assessed in patients with vestibular migraine and healthy individuals by ELISA. Migraine was induced in mice by NTG and HSP70 expression was examined in the trigeminal nucleus caudalis (TNC) tissue of mice treated with NTG and NTG together with exogenous HSP70. The effects of exogenous HSP70 on migraine-like symptoms were assessed through behavioral assays. Finally, the impact of HSP70 on oxidative stress and NF-κB signaling in migraine mice was investigated. Serum HSP70 in patients with vestibular migraine was significantly lower than that of healthy individuals. NTG administration significantly suppressed HSP70 expression in mouse TNC tissue which were reversed by exogenous HSP70. HSP70 alleviated NTG-induced mechanical hypersensitivity, light aversion and anxiety-like behavior. Finally, exogenous HSP70 suppressed NTG-induced oxidative stress and NF-κB signaling. Our study suggests that exogenous HSP70 may be a potential therapy for alleviating migraine symptoms and our promising finding warrants further investigation of HSP70 for clinical application.

更新日期：2021-09-02

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