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Fe–N–C Single-Atom Catalyst Coupling with Pt Clusters Boosts Peroxidase-like Activity for Cascade-Amplified Colorimetric Immunoassay
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-09-01 , DOI: 10.1021/acs.analchem.1c02115 Yifeng Chen 1 , Lei Jiao 1 , Hongye Yan 1 , Weiqing Xu 1 , Yu Wu 1 , Lirong Zheng 2 , Wenling Gu 1 , Chengzhou Zhu 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-09-01 , DOI: 10.1021/acs.analchem.1c02115 Yifeng Chen 1 , Lei Jiao 1 , Hongye Yan 1 , Weiqing Xu 1 , Yu Wu 1 , Lirong Zheng 2 , Wenling Gu 1 , Chengzhou Zhu 1
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Although single-atom catalysts with high enzyme-like activities have been found, the rational design of highly active peroxidase (POD)-like nanozymes is still a formidable challenge. Herein, highly active POD-like nanozymes were synthesized through loading Pt clusters on the Fe single-atom (FeSA–PtC) nanozymes. The POD-like activity of FeSA–PtC nanozymes is enhanced 4.5-fold and 7-fold, in comparison to that of FeSA and PtC nanozymes, respectively, which is attributed to the unexpected synergistic effect between Fe single atoms and Pt clusters. Based on the outstanding POD-like activity of FeSA–PtC nanozymes, a cascade signal amplification strategy was constructed by combining glucose oxidase for the colorimetric biosensing of prostate-specific antigens, exhibiting satisfactory sensitivity, high selectivity, a low detection limit of 1.8 pg/mL, and practical feasibility in serum sample detection. This work may serve as a tough foundation to guide the design of superior POD-like nanozymes and expand the application in biosensing.
中文翻译：
Fe-N-C 单原子催化剂与 Pt 簇偶联提高了级联放大比色免疫测定的过氧化物酶样活性
尽管已经发现了具有高类酶活性的单原子催化剂，但高活性过氧化物酶（POD）类纳米酶的合理设计仍然是一个艰巨的挑战。在此，通过将 Pt 簇负载在 Fe 单原子 (Fe SA -Pt C ) 纳米酶上来合成高活性的 POD 样纳米酶。所述POD状的Fe活性SA -Pt Ç nanozymes提高4.5倍和7倍，相对于Fe的SA和Pt Ç nanozymes，分别，这归因于单一的Fe原子和Pt之间的出乎意料的协同作用集群。基于 Fe SA –Pt C出色的 POD 样活性nanozymes，结合葡萄糖氧化酶构建了级联信号放大策略，用于前列腺特异性抗原的比色生物传感，表现出令人满意的灵敏度、高选择性、1.8 pg/mL 的低检测限，以及在血清样品检测中的实际可行性。这项工作可以作为指导设计优异的类 POD 纳米酶和扩展生物传感应用的坚实基础。
更新日期：2021-09-14
中文翻译：
Fe-N-C 单原子催化剂与 Pt 簇偶联提高了级联放大比色免疫测定的过氧化物酶样活性
尽管已经发现了具有高类酶活性的单原子催化剂，但高活性过氧化物酶（POD）类纳米酶的合理设计仍然是一个艰巨的挑战。在此，通过将 Pt 簇负载在 Fe 单原子 (Fe SA -Pt C ) 纳米酶上来合成高活性的 POD 样纳米酶。所述POD状的Fe活性SA -Pt Ç nanozymes提高4.5倍和7倍，相对于Fe的SA和Pt Ç nanozymes，分别，这归因于单一的Fe原子和Pt之间的出乎意料的协同作用集群。基于 Fe SA –Pt C出色的 POD 样活性nanozymes，结合葡萄糖氧化酶构建了级联信号放大策略，用于前列腺特异性抗原的比色生物传感，表现出令人满意的灵敏度、高选择性、1.8 pg/mL 的低检测限，以及在血清样品检测中的实际可行性。这项工作可以作为指导设计优异的类 POD 纳米酶和扩展生物传感应用的坚实基础。
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