Sulfur dioxide (SO₂) is an environmental pollutant in the atmosphere which is easily absorbed by the human body. After being inhaled in the body, SO₂ is quickly converted into bisulfite (HSO₃⁻), forming a balance in which SO₂ and HSO₃⁻ coexist in the body status. A large number of epidemiological studies have shown that abnormal levels of sulfite and bisulfite are related to the appearance of numerous diseases such as atherosclerosis, essential hypertension, and lung tissue fibrosis. Therefore, it is essential to develop an effective method to detect bisulfite. In this work, starting from 4-bromonaphthalene-1-carbonitrile, three uncomplicated but efficient HSO₃⁻ sensitive ratiometric fluorescent probes HNIC, CIVP and HVIC were designed and synthesized through ICT mechanism and the Michael-type addition reaction. The probes can image HSO₃⁻ in living cells. The probes not only have good fluorescence stability and strong anti-interference ability, but also display mitochondrial targeting ability.

二氧化硫（SO ₂）是大气中的一种环境污染物，很容易被人体吸收。SO ₂被人体吸入后迅速转化为亚硫酸氢盐（HSO ₃ ^-），形成SO ₂和HSO ₃ ^-在体内共存的平衡状态。大量流行病学研究表明，亚硫酸盐和亚硫酸氢盐的异常水平与动脉粥样硬化、原发性高血压、肺组织纤维化等众多疾病的出现有关。因此，开发一种有效的亚硫酸氢盐检测方法至关重要。在这项工作中，从 4-bromonaphthalene-1-carbonitrile 开始，三种简单但高效的 HSO ₃⁻灵敏的比率荧光探针HNIC、CIVP 和HVIC 通过ICT 机制和Michael 型加成反应设计和合成。探针可以在活细胞中对HSO ₃ ^-成像。该探针不仅具有良好的荧光稳定性和较强的抗干扰能力，而且具有线粒体靶向能力。