苯丙氨酸与基于油酸的囊泡膜相互作用。了解苯丙酮尿症背景下原纤维-囊泡相互作用的分子作用,The Journal of Physical Chemistry B

当前位置： X-MOL 学术 › J. Phys. Chem. B › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

苯丙氨酸与基于油酸的囊泡膜相互作用。了解苯丙酮尿症背景下原纤维-囊泡相互作用的分子作用
The Journal of Physical Chemistry B ( IF 2.8 ) Pub Date : 2021-08-22 , DOI: 10.1021/acs.jpcb.1c05592
Sourav Nandi ₁ , Biswajoy Ghosh ₂ , Meghna Ghosh ₁ , Souvik Layek ₁ , Pratyush Kiran Nandi ₁ , Nilmoni Sarkar ₁

Affiliation

在目前的贡献中，在光谱和显微镜研究的基础上，探索了在三个 pH 值（即 8.28、9.72 和 11.77）下油酸/油酸盐溶液的各种组件的表征和光物理学。通过使用香豆素 153 作为探针的时间相关单光子计数设置的皮秒溶剂化动力学实验，研究了组件内部和周围水分子动态响应的变化。一方面，执行时间分辨荧光各向异性测量以及荧光相关光谱实验，以提取有关这些组件内部限制范围比较的信息。另一方面，l-苯丙氨酸 ( l- Phe) 是苯丙酮尿症 (PKU) 紊乱的原因，而在形成囊泡的 pH 值下的油酸表明l- Phe 原纤维形态强烈地改变了由油酸形成的囊泡膜的动态特性。具体来说，l- 发现与油酸囊泡膜的 Phe 组件引入了囊泡膜的柔韧性并改变了膜的水合作用特性。为了跟踪原纤维诱导的油酸囊泡特性的改变，进行了各种光谱和显微镜研究。因此，实验输出的相互协调描绘了最先进的技术，它解释了原纤维诱导的油酸囊泡膜特性的改变，细胞膜的模拟设置，从而告诉我们这种改变在针对 PKU 等疾病的治疗调节剂的合理开发过程中，应积极考虑膜的特性。

"点击查看英文标题和摘要"

Phenylalanine Interacts with Oleic Acid-Based Vesicle Membrane. Understanding the Molecular Role of Fibril–Vesicle Interaction under the Context of Phenylketonuria

In the present contribution, on the basis of a spectroscopic and microscopic investigation, the characterization and photophysics of various assemblies of oleic acid/oleate solution at three pH values, namely, 8.28, 9.72, and 11.77, were explored. The variation in the dynamic response of aqua molecules in and around the assemblies has been interrogated by a picoseconds solvation dynamics experiment using a time-correlated single-photon counting setup employing coumarin-153 as a probe. On the one hand, the time-resolved fluorescence anisotropy measurement along with the fluorescence correlation spectroscopy experiment was executed to extract information regarding the comparison of the extent of the internal restricted confinement of these assemblies. On the other hand, an effort to investigate the cross-interaction between the self-assembled architectures of l-phenylalanine (l-Phe), responsible for phenylketonuria (PKU) disorder, and the oleic acid at the vesicle-forming pH established that the l-Phe fibrillar morphologies strongly alter the dynamic properties of the vesicle membrane formed by the oleic acid. Specifically, the interaction of the l-Phe assemblies with the oleic acid vesicle membrane is found to introduce the flexibility of the vesicle membrane and alter the hydration properties of the membrane. To track the fibril-induced alterations of the oleic acid vesicle properties, various spectroscopic and microscopic investigations were performed. The mutual reconciliation of the experimental outputs, therefore, portrays the state of the art, which accounts for the fibril-induced alterations of the properties of the oleic acid vesicle membrane, the mimicking setup of the cellular membrane, thereby informing us that alterations of such a property of the membrane should be taken into active consideration during the rational development of therapeutic modulators against disorders like PKU.

更新日期：2021-09-02

点击分享查看原文

点击收藏

阅读更多本刊新发论文